Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 3449228
End position: 3449299
Length: 72

DNA sequence
>NC_014814, from 3449228 to 3449299 (72 bp); Mycobacterium sp. Spyr1 CCTGACACCGAGCCGGAGGCGAGCATCGAGACCGAGCCGGAGGCGAGCATCGAGACCGAGCCGGAGGCGA GC
Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 3448133-3449233	Sequence	Mspyr1_33260 - hydrogenase expression/formation protein HypD
ORF. 3449226-3450347	Sequence	Mspyr1_33270 - hydrogenase expression/formation protein HypE