Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 3448133
End position: 3449233
Length: 1101

DNA sequence
>NC_014814, from 3448133 to 3449233 (1101 bp); Mycobacterium sp. Spyr1 GTGAAGTATCTGGACGAGTTCAGCAATCCCGAACTGGCGCGTCGCCTCATCGAGCAGATCGAAGCGGTCG CCACCCGCCGCTGGTCGATCATGGAAGTGTGTGGCGGACAGACACATTCGATCATCCGACACGGCATCGA CCAGCTGCTGCCCGATACCGTCGAGATGATCCACGGACCCGGCTGCCCGGTCTGCGTCACGCCGCTGGAG ATCATCGACAAGGCCCTCGATATCGCGTCGCGCCCGGAGGTGATCTTCTGCTCGTTCGGCGACATGCTGC GCGTGCCCGGCAGCGAGAAGGACCTCTTCCGGGTCAAGAGTGAGGGCGCGGACGTGCGCGTCGTCTACTC ACCCCTGGACGCGCTGACGATCGCCCGGGACAACCCGGACAGACAGGTCGTGTTCTTCGGCATCGGGTTC GAGACCACCGCCCCGGCGAACGCGATGACGGTGTACCAGGCGAAGAAGCTCGGCATCGAGAACTTCTCGC TGCTGGTCTCCCATGTGCTGGTGCCGCCGGCGATCGCGGCGATCATGGAGTCCCCGACCTGCCGCGTCCA GGCGTTCCTCGCGGCAGGCCACGTGTGCACGGTGATGGGCACCGACGAATACCCGCCGCTGTGCGACAAG TACGGAACACCGATCGTCGTCACGGGTTTCGAACCGCTCGACATCCTGGAAGGCATCCGGCGCACCGTGA TTCAACTGGAGGCCGGCCGGCACGAGCTGGAGAACGCCTATCCGCGCGCGGTCCAGGACACCGGCAACCC GGCGGCGAAGGCGATGCTGCTCGACGTGTTCGACGTGACCGACCGGTCCTGGCGCGGCATCGGCATGATC GCGAGCAGTGGCTGGCGGTTGTCGCCCGCCTACCGGCATTTCGACGCGGAGCACCGCTTCGCCGTCACCG ACCTCCACACCGAGGAATCGGCGATCTGCCGCTCCGGCGAGGTTCTGCAGGGACTGATCAAACCGCACGA GTGCACGGCCTTCGGGACCAGCTGCACACCCCGGAACCCGCTGGGCGCCACCATGGTCTCCTCCGAAGGC GCCTGTGCGGCGTACTACCTCTACCGGCGGCTGGAGGTCACCCATGCCTGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 3447819-3448136	Sequence	Mspyr1_33250 - hydrogenase assembly chaperone HypC/HupF
ORF.. 3448133-3449233	Sequence	Mspyr1_33260 - hydrogenase expression/formation protein HypD
ORF... 3449226-3450347	Sequence	Mspyr1_33270 - hydrogenase expression/formation protein HypE