Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 3238353
End position: 3241811
Length: 3459

DNA sequence
>NC_014814, from 3238353 to 3241811 (3459 bp); Mycobacterium sp. Spyr1 TTACTGCAGAACGGTACGCACTCGCCGCAGATCCAAGATCCCCGGCGCGCCCACATCGGTGGATTGGCGG GCAAGCTTCTCCCGCAGCGCCGCGAGGTCGACCGCACCGGGGGTGAACCCGGTGGCCTCGAAACGGCGCG CCCGGCGCGACTCGGCCTCCACCGCGTTGACCGGCGGGCTGTCGTAGGAACGTCCGCCCGGATGGGAGAC GTGATAGGTGCATCCCCCGCGTGACACCCCCGTCGCGGCGTCGATCAGCTCGAACCGCAGCGGACCGTCC ACCGTGATCGTCGGATGCAACGCGCTCGGCGGTTGCCAGGCGCGGTAGCGCACACCGCCGACCTGGACAT CGGGGTTGTCGGTGGCGAGCATCGGGACCGGATGACCGTTGACGGTGACGAGGTAGCGCGACCGGTCGGC TCCGATGAGACGCACCTGGAGCCGTTCGACCGACGAATCCACGTAGCGGGCGGTGCCGCCGGCCGTCGAT TCCTCACCCAGCACGTTCCACGGCTCGATCGCGCCGCGCAACTCGATCTCCACCCCGCCGAGTACGGCGG TGCCGATTCTCGGGAAGCGGAACTCGGTGAACGGGTCGAGCCAGCTGGTGTCGAACTCGACACCGTGCGC GCGCAGGTCGGCCGCGACCTCGGCGATGTCGTGAATCAGGAAGTGCGGCAACAGGTATCGGCCGTGCAGG TTGGCTCCGTGGCGGATCAGCGGGGCACGCAGCGGCTCGTCCCAGAACCAGGCGACCAGCGAGCGCACCA ACAGCGACTGCACCATCGCCATCTGGGGATGCGGCGGCATCTCGAACCCGCGCAGCTCCAGCAGCCCGAG TCGGCCCCGCGCGCTGTCGGGACTGTAGAGCTTGTCGATGCAGAACTCGGCACGGTGGGTGTTGCCGGTG ATGTCGGTGAGCAGATGACGAAGGGCCCGGTCGACGAGCCAGGGCGGAGCGGAGCTTGCGGAGCGACCAT GTTCCGGAGCGGAGCTTGCGGAGCTTGCGGAGCTTGCGGAGCGACCATGTTCCGGAGCGGAGCTTGCGGA GCTTGCGGAGCGACCATGCTCCGGAGCGGCGCCCCCCTCGGCCTTCGACAACCGCGCGATCTCAGCGAAG GCGATCTCGAGTTCGTAGAGGGCGTCCGGACGCCCCTCGTCCACCCGCGGCGCCTGGGAGGTGGTGCCGA TGAAACGCCCGGCGAACAGATAGGACAGCGCCGGGTGCCGCTGCCAGTAGGTGAGCATCGACACCAGCAG GTCGGGCCGGCGCAGCAGTGGCGAGTCGGCCGGGGTGACGCCGCCGAGGGTGATGTGGTTTCCGCCCCCG GTGCCGCCGTGGGTGCCGTCGACATCGAACGCCTCGGTCGAGAGCCGGGCCAGCCGGGCTTCCCGGTAGA GCGTCTCCAGCTGGGCGCGCTGCTCGACGAAACTCTCGGTGGGCGCGACGTTGACCTCGATGACGCCCGG GTCGGGCGTGATCGACATCGTGGTGAGGCGCGGATCCGGCGGCGGGCCGTAGCCCTCGATCACGACGGGG CAGTCGATCGAGGCCGCGGCGCTCTCGATGCGCGCGATCAGGTCGATGAAGTGCTCCAGCTCGTCGACGG GCGGCAGGAACACATACAGCAGGCCGTCGCGGATCTCACCGACCAGCGCCGTCGTCGGAACCCACTCGTC GTCGTCCGACGTCTCCTCCGGTGTCGCGGTGGCCGAGCGCGCGAGGGCGGCGCCACGTCCCAGCGCCGGG CGACGGTGGATCGGGTCGGCCTCGAACGTCGGCTTGGGGGGCTTCCAGCTGATCGAGTTCAGCGGCAGCC GCAGGCCCGCCGGTGAGTCGCCGTCGAGCAGCACGATGCGACCGCGCCGCAACGTCCAGTCCGCGCTGGC CCAGCCGGCGTCGTCGTCGCGGCGGTGCAGAGGCAGGACGAACGCCGTTGGTTCGCCGACCGATTCGTCG AGCCGCTGCATCAGTGCGGCCCGGCCCGCGGCGTCGTCTGCGGCCAGGTCGTCGTCGGCGGCGACCGGAG GCCCCTCGGGTCGGCGCACGGCTGCGATGAGCCGGGTCAGCGGGTCCTCGTAGGCGGGGCGGACCCGGTC TGCCGGCAGCCCGAGGCCGTCGGCGATCGCGGCCAGCAGCGCGCGGTGGGCATCGGGGGCGATTCCTCGA ACGCCGGGCGTCTCGGGCCACGGATCGGCAAGCAGCCGGTCATCGCTCCAGAGCGGTTCCCCGTCGGCGC GCCAGTAGAGCCCGATCTGCCAGCGCGGCAACGGTTCTCCGGGATACCACTTGCCCTGGCTGCGCTGCAC CAGGCCCTGCGGCGCCCACACCTTCTTCAGTCGCGCGGTCAGCGCCGATGCCCGCTCCCGCCTGTGCGGT CCGTCGGCGGCGGTGGTCCACTCCGGATCCACCTGGTTGTCGATCGAGACGAACGTCGGCTCCCCGCCCA TGGTCAGGCGAACGTCGGCCTCGCGGAGCCGTTCGTCCACCCGGCGGCCGAGCGCGTTGATCGCGGCCCA ACTGGCGTCCGTGTAGGGCAGCGTCACCCGCGGGTCCTCGTGCACCCGGGTCACGACGTTGCTGAACTCC AGGACGGTCTCACAGGGTTCGGTGGCACCGGTGATCGGAGCGGCGGACTCGGGATGCGGGGTGGCCGACA GCGGGATGTGGCCCTCCCCGGCGAACAGACCGGAGGTGGGGTCCAGCCCGATCCAGCCGGCTCCGGGGAT GTAGACCTCGGTCCACGCGTGCAGATCCGTGAAGTCCGCGGCCGGGCCCGACGGGCCGTCGAGCGCCTCG ACGTCCGAGGTCAGCTGGACCAGGTACCCCGACACGAAGCGCGCCGCCAGGCCGAGCTGACGCAGAATGG ACACCAGCAACCACGCCGAGTCGCGGCAGGAGCCGATCCCGGTGCGCAGCGTGACGTCCGGGGTCTGCAC CCCGGGCTCCATCCGCACCGAATAGCCGACGTCGGCGTTGACGGCGCGGTTCAGCGCGACGAGGAAGTCG ATCGTCCTGGTGCCCGTGGCCACGGAGAAGTTCGACACCCACGCGGCGGCGAGGTCGCCGGGGCCGGTGC CCTCCCCGTCCTCGTCGACGGGACGCAGATAGGGCTTGAGATCCTCGGCCAGCGCCTTCGGATACGCGAA GCCGACGCGTTCGGCGTAGTCCTCGATGAAGAAGTCGAACGGGTTGACGACCTTCAGGTCGGCGATCAGG CCGACGGTGATGGTCAGGCTGCGGGCGCGGGTCGGGAACACCAGCCGCGCGAGGAAGTTACCGAAGGCGT CCTGCTGCCAGTTGACGAAGTGATCGGCGGGCTCGACCTGTAAGGAGTAGGCCTCGATGGGTGTGCGCGA GTGCGGGGCCGGGCGCAGCCGCACGACGTGAGGATGAACCTCGACGAGGCGGTCGAATGTGTAGCTGGTG CGATGCTCCAGAGCTACCTTGATACCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 3235720-3238353	Sequence	Mspyr1_31110 - hypothetical protein (Protein is coded in complementary strand; from 3238353 to 3235720)
ORF.. 3238353-3241811	Sequence	Mspyr1_31120 - hypothetical protein (Protein is coded in complementary strand; from 3241811 to 3238353)