Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 3054084
End position: 3055508
Length: 1425

DNA sequence
>NC_014814, from 3054084 to 3055508 (1425 bp); Mycobacterium sp. Spyr1 TCAGAAGGGTGGCGGTTCGTAGGTTTCGGCGAGCCAGGCCTGGCGCTGACGTTCTTCCTCGGCGTCGAGC TCGATGCGCTGCCGCCGTTCACGCTCAATCCGGGCGCGGCGGTCCTGTTCGCGGGTTCGCTTGCGTCGCG GCATCATCGCCGACTTGTCCGACGGTTCCTGCGTCGAGGTCGGCGGCGGCAGCGGCGCCGTCGGTTCGGC GAGCTTCGGGAACAGACCGGCACCGTGTGCCTCGGTGCTGTACACGTGTCCCGTCGGGGCGATCACCACG ACGGTGCCATCGGGGCTCTGCGCATCACGCCAGCCGGACGGTCCGCCGTAGAAAGTCTTGATCAAATGAT GTGTCCTGCAGTAATGCTTGGTCCCCGACGCATGGGTGACTCCGAACGGCCACGGCGTCGTGTGGTCGAC ATCGCAGCCTTCCACCGGGGCGTCGCAACCCGGGAAGCGGCACGTCAGATCCCGCCAGCGCAGATAGTCA CGTAGCTTCGCCGACGGCTTGTACCCGGTCTCGGGTTCGGTACCGGGCTGGCTGACAGGGACCAGCTTCG CCGTCCTGGCCGCCGCGCGAACCTCCTCGGCCGGCAATACGCCGAAACCCGACAGGTAGCCCGGGTCGTC ACTGCGGCCCTCGACCGTCGCCTGCTCGGCCAGGATGTGGATGACCACCTTGGCTGCCGACTCACGCAAC GCGACGGCGGGGCAATCATCACCGCCGCACTGGCAGGCCAGCGTCGCCTCCCCGCGGCCCAGAGCGCCGG CAGCATCGGCGCGGCGCTGGTTCCAGGTCCGAGGGTCGTTGGCGCACACCGTGTCCGCCATGGCCTCCAG GCGCTGGTCCAGCGCCGCCGCATCTCGTGCCGTCAGCACGCCTCCGGCGAAGGCCATGCCGGGTACGTCC GGCGTCACATCGAAATACCGGTTGTCTTTGGCGGCCGGAGGTACGCGCACGCCTGCGGGGTCGAATTTGG CCACCCACTGATCCAGGCGATCTCTCAGCTTGTCTTTCGAAAACCGCATCCACTTGACGATGCGTCGGGC CAACGCCGCATCGAGCTCGGCGATGATCGCGTCGTCGACGGTTTCGGTGCGCGACAGGGTCAGCTGCACC ATGCGCTGGTCGATGTCGCCCCGGTAGAACACCTCCGCGACCTGAGGCAGGCGTTCATACAGCGACACCG CCATTCGTACTTGGCTTCCGGCACGCCAACGGCTGATGTTCTGCGCCGCCGACACTTCGGCGGCAACAGC TTCGAACACGTCGGTACGCCACAGACCCGCCTCGACGTACATGCTCTTGCGCACGCGGTACAGCGATGCG ACGGCCGCGAGACGCTCGGCAGCAGCGCGCGATTCGCACCGCGCAGCAGACGTCATCGCGTCCACGAGGT CTGCCGGACCCGCCGCTTCGAACAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 3054084-3055508	Sequence	Mspyr1_29400 - hypothetical protein (Protein is coded in complementary strand; from 3055508 to 3054084)