Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 30448
End position: 31899
Length: 1452

DNA sequence
>NC_014814, from 30447 to 31898 (1452 bp); Mycobacterium sp. Spyr1 ATGTCGTCATTGGTCAGCCCGGCTGAACAGCAGCCACAGTTGCGCCGCGAATTCTCCCTCGGATCGGCGT TCGCCTTCGCGTTCGCGTTCATCTCGCCGATCGTCGCCCTCTACGGGATCTTCGGTCTCGCACTGTCGGC GGCCGGGCCGAGCTTCTGGTGGGGCTTCGGGCTGGTCTTCGCCGGCCAGTTCCTGGTGGCGCTCGTGTTC GCGACGCTGGTTTCGCGGTGGCCGCTGGAGGGCTCCATCTACCAGTGGTCACGACGCCTGCTCGGCACCG CCTACGGCTGGTTCGCCGGGTGGGTCTACATGTGGACGCTGGTGATCGCGATGGCGACCGTGGCGCTGGG AGCGGCCGGCTTCACCGCGAACATCGTCGGCATCGAGGAGCCCTCCGGCGGCACCCTGGCACTGATCGCG CTGGTGATCCTGCTCGCCGGCACCGCCGTGAACCTGATCGGACGCCAGGCACTCAAGATCTTCATGATCG GCAGCATCATCGCCGAGGTCATCGGCTCGGTGGTGCTGGGCACGTGGCTGCTGATCTTCCACCGTCAGAA CTCGATCTCCGTGCTGTTCGAGGGCGGCGGCGCTGACGTCGACCTCGGGGCCTACATGGGCGGTCCGTTC CTGCTCGCGGTCGCGTTCATCGGCTGGTCGTTCGTCGGCTTCGAGAGCGCCGGATCCATCGCCGAGGAAG TCCACAACCCGCGCCGCGACCTGCCGAAGGCCGTGCTGTTCTCGATCGCCTTCATCGCGCTGGTCGTCGG CTACTCGGCGTTGGCGATCATCCTGGCCATCCCGGACCTCGACGCGGTGGCCGAGGGCGCCGTCGCCGAC CCGGTCTACGACACCCTGACGACGGCGCTGGGCGCCGGCATCGCCAAGCCCGTGCAGGTGATGTTCGTGA TCGGCTTCCTCGCGAGCTTCCTGGCGCTGCAGACCTCGGCGTCGCGCGTCATCTGGGCCTACTCCCGCGA CGGCGCGCTGCCCGCGGCGGGCACGCTGGTGCAGCTGCGCGGCAAGGCCAGGATCCCGGTCGCCGCGATC CTGGTGACCACCGTGATCGGTGCCGCACTGTTCCTGCTGAGCATCGTCGCCGGTGACATCTACTCGCTGA TGGTGAACTTCACCGCGGGCGGCTTCTACCTGGCGTTCCTGTTCCCGCTGATCGGGTTCCTGGTGGTGCT GCTGCGCAAGACCTGGAAGCCCGGATCGTTCTCACTGCGCGCGGCCACGATGCCGGTGGCCGTGGTCGCG GTGGTGTGGGCGGTGCTGCAGTTCCTCAACATCGCCTGGCCGCGAGTGGCTTTCGAGCAGCGCTACCTGG ACTGGTCGGTGTGGATCGGTGTCGTCGTGCTCGGCATCATCGGTGCGGTGTTGTACGCGAGTGTGAAGTC CCGCATCGTCGCGCCGCGGGATCACGATGAGCCGGCGCTCGCCGATGACTGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 30448-31899	Sequence	Mspyr1_00290 - amino acid transporter
ORF... 31874-32584	Sequence	Mspyr1_00300 - hypothetical protein