Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 3031480
End position: 3033294
Length: 1815

DNA sequence
>NC_014814, from 3031480 to 3033294 (1815 bp); Mycobacterium sp. Spyr1 TCATTTCGGCTCAAATGTCCTGGTCGCGGCCTGCTTGTCCTGGCGCTCGAGTAAGCGTGCATACCCCGCC CCGATGCCGAGCAGGGTCAGTCCGACGACGATGAACACGACGACCCGGAAGATGCCGTCGAGAGTCCCCA GGTCGAACAGGAACAGCTTGGCCATCGCGGCCGCCACCAGACCCAGACCGCCGCCCAGGGGCACCGACCG GTCCTCGCGCGGCAACCGTGCGGCATACCAGAACAGCACGGCGGCCATCGCGATCCAGAGGATCGTCGCG ACCATGTGACCGGCATAGAACCCGCCGTCACCCCCGATGACGAGCATGCCCGCGGTCACCGTGCACGACG TGACGGCGTAGACCGTCACCACCGCCACACCGACCCACAGCGCGCTCTCGGTGCGGTCCCACATCCACAC GATCGTGACCGCGGCGGTGGCCAGCAGCAGGCTGGAGACCAGGGTCGAGATCCCGGTGGCGGTGCTGACC TCGATCGGTTGGAGCAGTACGGCGGGCGGTGCATAGCTCAGGTGGGCGGCGGCGCCGATGACGGCGAATC CGATTGCCACCCAACGACCGACGGCATCCCGTCCCACCGCGACGGCGACCACGACCGCCATCGCCAGCAG CACGGGTCCGCCGACCCTGCCGTCGAACGCGGTCGTCACCGCGATCAGCGCAGCAATCGCCGATGTCACG GCGAACACGCGACGAACGACTCCGTCGACCCCCGGAAGGCGGTCAGCGAGCAGGGCCACCGCGAGCAACG CAGCGGCCAGTGCGGCGGCCATCAACGCGGCGACGACGCGGTCCACCGCGAGGTTCACGCACAACACCGG CACGATCCCGGCCACCGTCAACAGGGCCATCACGACCCGATTCCCGGTCCCGGGCAACAGGAGCAGCGCG GCGGTCAAAGCGATGACCGCGGCGATGCCGCATGCGCCGGCCAGCCAGAGGTCCTCTCGCGAGTCGATGT GACGGGCCGCCAGCGCCACCAGCAGCGGCAGCACGGCCGCCGCGATCCGCGCGGCATGCAGCCAGATCCA GTCCTTGCCGAGCTGCACCGGTAGCGACACCGCCGCCAGCGCCAGCATGAACCCGACCAGCAGCAGGGAC ACCCCGTCCGTGACGATCGGGGCCAAGACGATCAGCGGCACCAGCACCAGCAGCCCGAGATGCTCGGAGT CCCATCTGCGGGCCAGCGTCAGCCCCGCGCCGGCGACCACTGCGGCGAGCACGAGTCCGGCCGGCGCCGG CACCCAGTCGTAGATCGTCGTGACCGCGATGACGTCCATGTAGGCCGCGGCGACACCGGTCGCCACCAGC GCGATCGCCCCGACCCTGCCGCCTGGCCGGGTGTTGAGCCACCATCCCGCACCCACCAGCGCGGCGGCCA GCGCGCCGCCGGCCGAGACCCGGAACTCGGGGCGCAGGATGCCGGCCTGCGCGGCGAGCACCAGAAGCAG GACCACGCCGATCAGGGTGACCGCGACACCGGCGACGGCCAGCAGCTTGCCGATCCAACCCTCCGAACGT TCCTTGCGCGGTGCCGGTGTCAGTGTCGGTGTCGGTGTCGGTGCCGGCGCCTGTGGCCACGGCCGGAACG GGGCGGGCTGCGGCGGATACTGCGGCCAGTACGCAGCGGGCTGGGGCGCCGGCCGAGGCGCCGCAGCGGC CGGCGCCCGGTCGGTGAGGATCCGGTCGAGCGCGGCGAGATCGCCGGAGACGCGAGTGAGCTGGTGGGAG ATCGCGGCGAAATCCGCGGAGAGCCGGGCGATGACGGCGGAGTGCGGTTCGGTCATGTCCGCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 3031480-3033294	Sequence	Mspyr1_29220 - hypothetical protein (Protein is coded in complementary strand; from 3033294 to 3031480)