Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 298694
End position: 302026
Length: 3333

DNA sequence
>NC_014814, from 298694 to 302026 (3333 bp); Mycobacterium sp. Spyr1 CTACAGTCGGCCCTCGCGACGCAGCAGGTCCTGCGCGCTGACACCGGAGCCACCGCGGCGCGGTGCATCG GGATCGGGCCGGGTGTTGAGCTTCTCCGTCGCCGCCTCGGCGTCCGGCTTGCGCTGCGCCGGGATGGCCC GGGTGGCGGTGTCGTCGGGCTCGTCGTCGGACGCGGCGTCCTCACCGCTGTCGGGCATCGCCCGGGTCGG TTCGGCCGACGGGCGCATCGGCTGCGGCGCGCCGGCCGGGCCGGTGGTGCCGCCGCCGCGCAGGTCGCCG AGCCACGACTCGATCTCCCGCTCATCGCGGGCCGGAGAGCCCGACGGCGGCGGTGTGGGAGACGGCGGTG TGGGAGACGGCGGAGTGGGAGACGAGGACGGCATCGCGCGCTGCGCTGCGGCCGCGGCTCCGCTGACCGC GTTGCGCACCGCGTTCTTCGCGATCGACAGCCGGGTCGTCTGCGGCTCGTCCTGCGCCGGGGGCGGAGGA GTAACGGGTGGCCGCGGCGGGGCGGTCGGGATGCGGGTGGTCCCGGCGGCCGCCGGGTCCGGGTACGTCG GGGTGCCCATCCGGGTGGTGACGGCGGGAGGCCGCACCGGCGCGGGACGTCCCGGCGCGGGATGCGTGGG GTCGTGCGGCGGCAGTGCGCGCGGCGGCGCCATCACCGGGGGTCCGCCGGCGCCGACGAGCGCCTCTGCG GGCGCCTCGCGCTCCTCGCGCACGGCGGGACGCTTGCGCTCGTCGGGCAGTTCGGTCTCGCCGAGGCCCA GCTTCTCCTGGATGCGCTTCATCCAGCGCGGCGCCCACCAGCAGTCGTCGCCGAGCAGCTTCATGATCGC CGGCACCAGGAACATACGGATGATCGTCGCGTCCAGCAGCAGCGCGATGAGCAGGCCGAACGCCAGGTAC TTCATCATCACCAGGTCGGAGAACACGAACGCACCCGCGACCACGGCCAGCACCAGAGCCGCGCCGGTGA TGAGGCGGCCGGTGGTCGCGGTGCCGATGCGGATGGCCTCGGCCGTGGAGAGCCCGCGCTCGCGGGCCTC GACCATGCGGGAGACCAGGAACACCTCGTAGTCGGTCGACAGACCCCAGATCACCGCGATGATCAAGCCG ATCATCGGGGCCATCAGCGGCTGCGGTGTGTAGTTCATCAGCCCGGAGCCGTGGCCGTCGACGAACATCC ACGTCAGGATGCCCATCGTCGAGCCGAGCGTCAGGGCGCTCATCAACGCCGCCTTGATCGGCAGCACGAT CGAGCCGAACGCGAGGAACATCAGGATCGTCGTCGTGACGATCAGCACCAGACCCATCAGCGGCAGCTTG TCGAACAGGCTGTGGATGCTGTCCTGCTCCAGCGCGGGCGTGCCGCCGACCGAGATGTCCACACCGCGTG GCGGCTGGATGGAACGGAGCTCGTCGATCTTCTGTGACGCGTCGTTGCGGTCGACCAGTCCGTTCTGCAG CACCCGGATCGACGGATCCTTGGTTCCCGTGTCCTGGGCGGCGCGCTCCTGCCACATCTTGGACGGATCG TTGTCCGGATCGATGAAGCCGGGGACCGTCATCGCCTTCGCGCGCACCTCGGCGAGCTGCTGGTCGGTGA CCGGCTCGCCGTCCTGGCGTTCGATCACCAGCGTCAGCGGCTCGGTGCGGAAGCCGGGGAAGGTGCGGTC GAAGTCCTCCTGCGCCGTACGGACACCGTTGTCCGGCGGCAGATACTTCTCGCTGATACCGCCCAGCGCC AGCTGGCCGAGCGGCAGGATCAGCAGGATCATGCCGATCAGGATCGGCGCGGCGAATGCGATCGGCCGCT TCATCACGACGTTGACGAGTTTGCCCCAGAAGCCCTTCTCGACCTCGGCGCGCGTCTTGGTCTTCTGCGT CTTCTCGGCCAGCCACTCGACCCACCACACGATGGGACCGCGCGCCAGCGGGACGTAGCGCGCGAGTGCC AGGCCTCCGGCGACGACGGCGACGAAGATCACGACGCCGATGAGGACCCAGTACAGGCCGTCGCCCAGGG ACTCCCTGTTGGCGATCGCGAGGTAGGCGATCCAGCCGAGCAGCCCGACGTAGCCCAGGGCCAGGCCGAT CATGGTCAGCGGCAGCCCCTGCTCACCGGTTTGGCGGATCTGCTTGCGGGCCCGGTGGCCGAGGAAGATG CCCACCGGCGGGAGCAGCAGGCCGGCCGGGATGGAAGCGATGGCGAACTGGTTGGTGCGGGTGTTCGAGG GATCCGAGGACACCTCGTTGTTGACGAACTTCAGCAGCCAGCGGACGCCGAGCGCGTCGACGCGGGGGCC GAGGATGGCCAGCGCGGCGGGCAGCACCGTGACCGACAGGATCGCCGCGAGCATGACCGACGCGATGATG GCGTAGGTGATCGACTTCAGGAAGCCCTGCGGGAACAGCAGCAGCGGTACCGAGGACGCCACCAGGATGA CGGCCGAGAACATGACCGTGCGGCCCGACGTCATCACGGCGCGACGGACGGCGGCCTCGGTGTCGTAGCC CTCGGCGATCTCCTCACGGAACCGGCTCACCATGAACAGGCCGTAGTCGATCGCGATGCCGAGCCCCATC AGGGTGACGACGGGTTGGGCGAAGAAGTGGACGGGCATGAACTCGGCGAACACCCGCATGATGCCGAGCG CACCCGCGATGGTCAGACCGCCGATGATGCCGGGCAGGGCCGCGGCGATCACGCCACCGAAGACGAAGAA GAGCACGACGCAGACCAGCGGGATCGCGGCCACCTCGGCGCGCTTCTGGTCCTCGCCGATGGTCCCGGTC AGCTCGCTGGCCAGCGGTTGGAGTCCGGCGAGCTGGATGCCGCCGTCGTTGACCTGGGACAGCTGGGGTT CGATCGCCTGGTAGTTCTTCAGGATCTCGTCGTCGTTCTCGCCCTTGAGCGGGATGCTGACGAAGGTCTT GGACAGATCCTCGGTCTTCATCTGCTGCACGGTCTCGGCCGCCGCGTCCGGTGCGCGCAGCCAGCCGACC CAGCTGACGATCCGGTCCTCGTTCTCGGCGACGAGATCGTTGAGCTCGCCGGTGACCTTTTCCATCCACT CCGGGTTGTCGACCTTCTCGCCGTCGGGCGGCGTGAGGATCGCCACCACGTGGCTCGTGCGGTCGCGGCC GTAGGCCTCGTCGGACACCAGCGACGCATGGACGGACTGGCTGCCCTCGTCGTAGAAACCGCTCTGGGTG ACGTGCTGTCCCAGGCTCATGCCGTAGACGCCGCCGCCCAGGCACAGTGCGACCATGACACCGATCACTA TGTATCGGTACTGGTACACCGTTCGACCCCACCAGGCGAACAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 298694-302026	Sequence	Mspyr1_02990 - hypothetical protein (Protein is coded in complementary strand; from 302026 to 298694)