Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 2817711
End position: 2823206
Length: 5496

DNA sequence
>NC_014814, from 2817711 to 2823206 (5496 bp); Mycobacterium sp. Spyr1 TCAGTTCTCGACGCTGTCGAACAGCGAGTCGAGCAGGCTGTCTCCCGAATCCTCCTCGGCTGCCTCCGCG ACCGCCTGGGGCGCCACCTTCTCGGCGAGGTAGGCCGCAAGCGCCCCGATGGTCGGGTAATTCCACAGCA TCGTCGCCGACAGCTCGATCCCGGCGAGCTTCTCCACCTCGCGCCGAATCGACATCGCCATCACCGAGTT GAGTCCCATCTCGGCCAACGGACGGTCGGAATCGACCTCGTCGTCCCCGATCCGGAGCTCGACGGCCAGG ATCGTGCGCAGACCGCTCTCCAGCTCGGAGACCACGTCCTCGGCCGACATCTGCGACCAGTCGCGCTCCG GTCTGGACTCGGCGGCCTGATCGGTGTCCTCGTCGGCGTCCAGCGTCGGCGCCATCACCACGTGGGGGAC GTCGAAGCGGCTCGCGTACTCCCAGGCGGCGAAGGCCTCCGCCGGGGTGACCGGCCGAGACCCCTGGCGT TCCAGTTCGTCGATGACGACCTGAGCGTCGGATCCGAAGCCGAGTCCGCGCCACGCCACCCAGTCCAGGC TCGCCGTCACGCCACCCTCGCGGTGCCGTGCCCGGGCCAGTCCGTCGAGGTAGGCGTTGCCCGCGGCGTA GGCACCCTGCCCGGGCACACCGAACACGGTGCCCGCCGAGGCGGTCAGGTACAGGAAGTCGACGCTGTCG GTCGGGAACAGTTCGTGCAGCACCCGGGCGCCTGCGACCTTCGGCCACAGCGTGCGGCGCAACCGCTCGT CGTCGATGTCGGTGAGCAGCTGGCTGTCGGTGAGCCCGGCGCCGTGGATCACGCCGCGGATCGGCGGGGC GCCCTGCTCGTCGCGGCGGGTCAGCAGCGCCTCGACCGCGTCGCGGGAACCGATGTCGACAGGTGCGAGC TCGACGGCGACGCCACGCAGTTCCAGCGCCCGCACCGCGGCGATCTTGTCGCGGGCGTCCTTGTCGAGAT CGTCGTCGTCCCACCGGCGCCGCGGCGGCAGAGCCGTCCGGCCGGCGAGGACCACGCGGCGTGCGCCCCG GTCGGCGAGCCACTCGGCCGTCACCAGGCCGAGTGCGCCCAGGCCGCCGGTGATCAGATAGGCGGCGTCG GGACGGCAGCGCAGGGGCTCGCGCACCGGGTCGGACGCGAGGGCCGCCAGCTCGGGGGCGAAGAACTCGC CGCCGCGCAGCGACAGGATCGACTTGGCCGCGACCGGCAGGACACCGGCGAGGGCTTTGGCAGTCTCGTC GAGGTCGGCGCCGGATGCGACGTCGACCAGCCCGCCCCACAGCTGCGGCTGCTCGGCGCCGACGACGCCG GCGAGACCCCACAAGCTGCTGTGCGGCAACGACTGCGCCGATTCGGCGTCCCGCACACCCTCGGTGAGGA TCCACAGTCGCGGCTCGCCGGTGCCGGGGCGGTCGGCGAGTCGGGAGACCAGCGAAGCCACCTCGCCGCA GAGCCGGACCGTCGCATCGAGATCGTCACCGTCGGGGCCGGGTACGTACAGCACGCACTGCGCGGTGGCC TCGTCGGCGTCGGTGTATCCCGCGGCGGCGAGGCGCTCCCGCAGGGCGTCGGCGGTGGTGCCGGAGCCGA CGACCGCGAGGGACGCGCCGGCCGGCGCAGTCTCGGACGGGGCCGTCCAGGGTCGCCAGGCGATCTCGTG GGCGACGCTGCGCGGATCGGCGGGCGCCGCCTCGGCGGGCCCTGCCTGCACGTCGACGTAGCGCAGGCCG ACGATGTCGACGCGGGTGCTGTCGTCGGGGACGGTGACCGCGACGTCGAGCACCAGATCCTCGCCGGTGC CGGGGCGGCGCCGCACGGTGACGGTGCCCGAGGATGCCAGCAACTCACCGGTCGCGGTCAGGCTCTCCAC CGCCGCCGGCACGAGGAGGCGGTCGTCGTTGATGTCGGCCAGGCGGGCGACCTGGACCGCGGCGTCGAGC AGCGCCACCGCGGACCCGTCCTGCCACCGCACCTCGGCGAGCACTCCGTCGCTCGTGGCGCGGCAGGTCT CGATGGACCACGGGAAGGGCTGCCCCTCCACGCCCCACCGGGACTGCAGCTCCTCGACCGTCGAGCGGTC GTAGTCGGTCGCGGCGGTGACGTCGACCGCGCCGGTGTCGGCGGGACGCGGCTGCGCGACCCGCGCGGTG GCATGGCGCACCCAGCGGTTGGCCGGGGCGTCGGCGTGGGGCGCCGAGGCCATCGTGATCGTGTCGCCGT GGGCGACGACCTGGATGACCTGCGGCTGGTCGACCACGACCGGCCGGTCGAACCGAATGTCGGCCAGCGC TGAGGCGCCCGACTCCGCCGCCGCGGCCGCCAGCGTGCGCAGCAGCACCGACATCGGGACCACCTCGACA CCCTGGATGCGGTGGAACCCCGGGTACGGCTTCGCGTCGCGTACCAGCCGGGCCTGCCACAGATGGGTGG GCGGACTGGTGGCCACCGACGTCAGGTCGCCGAGCAGGGTGCCCGGTCGGGGCGCCGACGGGCCGCGACG GTCGACCTTCTTGTGCGCGACCCAGTGCCTGGTGTGGTGCCACGGCGTGGCGGGCAGGACCGGGTGCGGT TCCGGACCGTGGTCGCTGACCGGGGGTTTGGTCGTGAACGTGCTGTTGAGGTTCGTGTGGAACGAGAGGG TCTCGTCGGGGTTCTCGTCCGTCTCGCGCAGCAGTGTGGGCACGCTGTGGTGATGGCTCGTGCCGAGCGT GTCGCTGATGCCGTAGGTCAACAGCGGGTGCGGGCTGACCTCGATGAACGTGCCGATCTCCGCCCCCGCG GCGGCCACCGCCTGCTCGAACCGCACCGGGTTGCGCAGGTTGTCCACCCAGTAGTCGGCGTCGAACACCG GCTCGGGACCGTCGTGCTCGCGCGTCGTGATCACGATCGGGATGTTGGGAGACACCGGGCGCAGACCGCT CAGCGCCTCGCGCAGCTCGGGCAGGATCGGGTCGATCGTCGGGTGGTGCGACGCCACGTCGACCTCGATG CGGCGTGCCAGCCGGTCCTGCGCGGACACCGCGGCGATCACGGCGTCCACCTGGTCGGGCGGACCCGCGA TCACGGTCTGGCGCGGCGAGGCGTACACCGCGAGCGTCACGTCCGGGTAGTCGGCGATCAACGCGGTCGC GGCGTCGGGATCGAGTTCGATGAGCGCCATCGCTCCCTGACCGGACAGCCGCGACATCAGCTTCGACCGG GTCGCGATCACCTTGAGCCCGTCGGCGACCGACAGCGCGCCGGCGACCACCGACGCGGCGACCTCACCCA TCGAATGTCCGATGACGGCGTCGGGGTGCACGCCGTAGGCGCGCCACAGTTCGGTCAGCGCCAGCTGCAT GCCGACCAGCACCGGCTGGATGCGGTCGATGCCGGTGACGGTCTCGCCGCCGGCGATGATCTGCTGCAGC GAGAAACCGACCTGTTCGACGAAGACCGGTTCCAGCTCGGCGACGGCCGCGGCGAACGCCGGCTCGTCGG CCAGCAGCCGCCGGCCCATGCCGGTCCACTGCGCGCCCTGGCCGGAGTAGACGAACACCTTGCCGGGCCG GCACGAGCCCTGATGCGGAGGGACGACTCCCGGGGCGGTGTGTCCCGCCGCGAGCGCCTGCAGGCCGGCC AGCGCGCCCGCCCGGTCCCGCGTGCAGACCGTCGCGAACTTCGAGTGCTGGGCCCGGTGGTGGTTCAGCG AGTGGGCGACCTGGTCCAGGGGCACGTCGGCCCCGTCACCGGCCAGCCACTCGGCGAGCATGCCCGCGGT CGTCGCGATGCGCGGCGCCGACTTACCGGCCACGACGAGCGTGGTCACGGGTGATGCCGCAGCGGGCGCG ACGTCGGTGTCCTCGGCGGGCGTCGGGTCGGGCGCCTGCTCCAGGACGACGTGGGCGTTGGTGCCGCCGA AACCGAACGACGACACCCCGGCCACCCGCGGGCGGCCGACGGGCTCCCAGTCGGTGACCTCGTCGACGAC CTTCAGCCGGAGGTTGTCGAACGGGATGTGCGGGTTGGGTGACTGGTAGTCGAGGTTGGCCGGGATCCGG CCGCGCTGCAGCGCGAGCACCGTCTTGGCGAAGCCGGCGATCCCGGCGGCCGCCTCGGTGTGCCCGAGAT TGGACTTGATCGCCCCGATCAGCAGCGGCGCGTCGGCGGCGCGGCCGCGGCCCAGCACGCTGCCCAGGGC ACGGGCCTCGATCGGGTCGCCGAGCAGGGTCCCGGTGCCGTGCGCCTCGATGTAGTCGACCTCGCGGGGT TCGACGCCCGCGGCGGCATACGCCGAGCGCAGCACCGACATCTGGGCCGCCGGGTTCGGCGCCATCAGCC CGTTGGACCGGCCGTCCTGGTTGACCGCGGACCCGCGGACCACGGCGAGCACGCGGTCACCGTCGCGCTG GGCGTCGGACAGCCGCTTGAGCACCGCGACACCACAGCCCTCGCCGCGCACGAAACCGTCCGCGCGCGAG TCGAAGGCATGGCAGCGGCCGGACTTCGACATGGCCTCGGCCTGGTCGAAGCTGCGTGTCACGGCCGGCG ACAGGATGACGTTCACCCCGCCGGCCAGCGCCACGTCGGAGTCCCCGCTGCGCAGGCTCTGGCACGCCAG GTGGATCGCCACCAGCGACGACGAGCACGCGGTGTCGATCGTGACCGACGGGCCGCGCAGGTCGTAGAAG TACGACACGCGGTTGGCGATGATGCTCAGCGCCCCACCGGTGCCGGAGTACGCGTTGACCTCGGCGAGAT CCTTGGACGCCATCACGGCGTACTCGCCGAGGCAGGCGCCGACGAACACGCCGGTCTGGGTGTGGCGCAA CACGTCAGGGCGGATCCCGGCGTGCTCGAGAGCCTCGTGGGTCACCTCCAGCAGCAGCCGCTGCTGGGGG TCCATCTTGTCGGCCTCGCTGGGCGAGATCTCGAAGTAGTCCGCGTCGAACTCGTCGATCTCGGAGAGGT AGGAGCCCCAACGGGTGGTGTGGGCCAGCGCGGCCGCGCCCTCGGGAGAGCCGTCGTCGTACCAGGACCA CCGGGTCGGGGGCACCTGGCTGACCGCCGAGCGGCCCTGCGCGAGGAACTCCCAGAGGTCGTCGGGACCG TGGATGTCTCCGCTGTCTCCGATGTCTCCGATGTCTCCGCTGTCGTCGCCGCCGCCGGGGAACCGGACGC CCAGGCCGACGACGGCGATCGCGTCGTCGGACACCCGCCGGATGGGCGACACCTCCGCGACGGCCTCGAC CTCACCGCCGGTCAGGAACCGGGCCAGCGCGTTGATCGTCGGGTACTGCCAGAACTCGACCGGGGACACG GTTCGGCCCAGCAACTCCGACAGCTCTCCGGTGAGCACGACGGCGTCGCTGGATCCGACGGCGAGATCGT TCAGTGGTGCATCGAAGTCGATCTCATCAGGACTACATCCGATGTTGGTGACCAGATAGTCGACCAACCA GTTCCGAATGGCGTCCTCGTCGAACGCAGAGGTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 2817711-2823206	Sequence	Mspyr1_27260 - polyketide synthase family protein (Protein is coded in complementary strand; from 2823206 to 2817711)
ORF... 2823203-2824981	Sequence	Mspyr1_27270 - acyl-CoA synthetase (Protein is coded in complementary strand; from 2824981 to 2823203)