Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 2554543
End position: 2555028
Length: 486

DNA sequence
>NC_014814, from 2554543 to 2555028 (486 bp); Mycobacterium sp. Spyr1 CTAGTTGACGACGTCGTCGTCGGTACGTCGCTCCGCGATGGCGGCCAGCCTCCACGTGGTCTCGCGGTTG CGCGGAAAGACCCCCGCGAGAGCCAGGGAGTCGGGGAGCCACTTCATCGGGACGGACACCGGCACCTCGG CCATCTCGATCAGACATCCGCCGCCGTCCAGATCATGGAGGCGCAGCGTGATCCGGGCTTTGCCGAGGGG TCGCCCGTTCGCGATCAGGACGAGTTCGCGCTCGGGCGTGCACGACTCGACCACGGTGCTGTCGTTGATC ACTGCGGGCCACACCCCGATCGAGTGATGGATCGTGCTGCCGGGCTGCGGCCAGTCCGGATCGACGGCCC GCATGCGGCTGTTGCCCACCACCCACTGGGAATACGTCCAGCCGTCGGCCAGGACGGACCACACGCGTTG CCTCGACGCCGACGTCTCGCGGCGCACGATCGGTGAACTGGTGATTTCGTCTGGTTCTGTCGCCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 2554543-2555028	Sequence	Mspyr1_24680 - hypothetical protein (Protein is coded in complementary strand; from 2555028 to 2554543)