Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 245528
End position: 246727
Length: 1200

DNA sequence
>NC_014814, from 245528 to 246727 (1200 bp); Mycobacterium sp. Spyr1 TCAGTTGTGTGAGGTGCGCCAGAAGTCGTCCCACGCCGACTTGTTGCGGTAGTCGCTGACCAGGTCGGCG CTGCGCAGCGGCGCCATCTGGTCGAGCAGTTCTGCCACACCCGACGGGTCCTTCGGCGCGGGCACCTCAG AGTTCGAGGAGGGCTTGCCGTCGGCGAGCTGCAGGGCGAAGCCTTCCTCCGTCAGCATGTAGTGGATGTA GAGCTTCGCGGCATTCGGGCTGTCCGTCTTCGATGCATACGCGATCGCCGACGGGAACGACTGCAACGTC CAGGGTTTCATGCCGTCACACAGCGCCAGGTGGTAGCCCTTGTCGGCGTTGTTGCGAAACTTCGCCATGG ACATCGGTCCGAAGCTCGGCTCCTTCTGGTCGGGGGCGCCGACGGCTTCGGAGACCTCCTCGTCGGTCGG GGTGATCATCGGGTTGTTCTGAGCAAGGCGTTTGACCCATTCGTGGGCGGCGTCGGGCTGGTCGGTCTCG AGGTCCGTGCCGAAATGCTGCGTGTAGGCCTCCCTCAGCGCGGCGTCGTCGTTGAGCGCCATGGAGTTGA ACCAGTAGGGGTACTTGGGATTGCTCAGCGGGTCGGCGATCGCGACGCGTCCGTTGAACTCCGTATCGGT GAGCTGCCAGATGTTGGACACCGGGCAGCTGTCGTAGGTCTGGCTGTTGTAGGCCCAGCCCATCGGGCCA TAGTGGACGACCAGCGGGTACTGATCCTCGGGGTTGATGGTGTCCTTCATGTCCCCGGGCACCCAGTTGA CGAGGACACCCTGATCCAGCAGCTCGACGGCGTAGGACGGGAGATCCTCGTTGACGACGACGTCGCCGAT GACATCGCCGGACTCGTTCTCCTGCAACACCTTGTCGATGACGCCGAGCTCGACCTTCACGCCGGTTGCG TCCAGTCCGTACTTGGCGGCGAAGGCTTTGGCGGTGTCGACGACCTTGCCGGTCTGGTCGTAGACGGTGA TGCCGGGCTCGGTGCGGGCTGCGGCGATCAGCGCGTCGAGATCGAAGTTCGCCGGGTCCTCGTCGGGTTG GGTCGTCTTCTGCGCCAGTGGCGACGGCGTGGTGCCGGTGCCGGTGCCGGTGCCGGTGGTGGGGTCGGAC GGCGCGCAGGCGGTCACCAGCAGGGCGAGGACGGCGCCGGCCAGCGTGACGCGCCGCATCCGAGAAAGGT TGGTATGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 245528-246727	Sequence	Mspyr1_02550 - ABC-type Fe3+ transporter periplasmic protein (Protein is coded in complementary strand; from 246727 to 245528)