Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 2212642
End position: 2213940
Length: 1299

DNA sequence
>NC_014814, from 2212642 to 2213940 (1299 bp); Mycobacterium sp. Spyr1 GTGACCAACGTCGGACAACTGATCAGCGCGATCGTGCTGGTGGCCTTCGGCGGCCTGTTCGCCGCCACCG ACGCCGCGCTGAGCACCGTCTCCATCGCACGCGTCGAGGAGCTGGTGCGCGAAGAGCGGCCGGGCGCCGT ACGGCTCCTGAGGCTGATGGTCGACCGGCCCCGCTACATCAACCTGATCGTGCTGCTGCGCATGACGTGT GAGGTCAGCGCCACCGTGCTGATGGCCGCCTACCTCGACGACTACCTGGGCGTGAGCCGGGGGCTGATGG CCGCGGCCGCCATCATGACGGTCGTCAGCTTCGTCGTCGTCGGCGTCGGGCCCAGGACCCTGGGCCGCCA GAACGCCTACACCATCGCGTTGGTGACAGCCCTTCCGCTGCAGGCGATCTCGGTGGTGCTCACCCCGATC AGCCGGTTGCTGGTGCTCATCGGCAATGCGCTGACGCCGGGGCGCGGTTTCCGCAACGGCCCGTTCGCGT CGGAGATCGAACTGCGCGAGGTCGTCGACCTGGCCCAGCAGCGCGGCGTCGTCGCCGACGACGAGCGCCG CATGATCCAGTCGGTGTTCGAGCTCGGGGACACCGCCGCCCGCGAGGTCATGGTGCCGCGTACCGAGATG GTGTGGATCGAAAGTGATAAAACTGCAGGTCAAGCGACCTCCTTGGCGGTGCGAAGCGGACACTCCCGGA TCCCTGTGATCGGGGAGAACGTCGACGACGTCGTCGGCGTCGTGTACCTGAAGGACCTCGTCCAGAAGAC CTACTACTCCAACAACGGCGGACGCGACACCGCGGTCTCGGCGGTGATGCGCAAGCCGGTGTTCGTGCCC GACTCCAAGCCACTCGACGCGCTGCTGAGCGAGATGCAGCGCGACCGCGTGCACATGGTGTTGCTTGTCG ACGAGTACGGCGCGATCGCGGGGCTGGTGACCATCGAGGACGTGCTGGAGGAGATCGTCGGTGAGATCGC CGACGAGTACGACACCGACGAGGTGGCGCCCATCGAGGATCTCGGGGAATTGTCCTATCGGGTCTCGGCA CGTCTGCCCATCGAAGACCTCAGCGAGCTCTACGGCATCGACTTCGACGAGGGTCTCGACGTCGACACCG TGGGTGGCCTCGTCGCCCTCGAGTTGGGCCGGGTGCCGCTGCCGGGCGCCGAGGTCGAATGGGACGGTCT GCGGCTGCGCGCCGAAGGTGGGCCCGACCCGCGCGGCAGGGTCAGGATCGGCACCGTGCTGGTCAGCCCC GTCGAACCACCGAACGATGACGACGAAGGACACGAATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 2212642-2213940	Sequence	Mspyr1_21450 - hypothetical protein
ORF... 2213937-2214263	Sequence	Mspyr1_21460 - hypothetical protein