Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 2198209
End position: 2202588
Length: 4380

DNA sequence
>NC_014814, from 2198209 to 2202588 (4380 bp); Mycobacterium sp. Spyr1 GTGACGGCGGCGAAGACCGGCGCCATCGCCGATGTGATGGCGCTCAGCCCGCTGCAGCAGGGGTTGTACT CGCTCACGGCGATGAGTGACGGCGACGACCCGTACCTGATCGGGATGAGCGCCGACATCACCGGCGCACT GGACGTCTCGCTGCTGCACGACTGCGCCGCCGCGGTGCTCGCACGCCATCCGAACCTGCGCGCCGGGTTC TTCCAGGGCGACCTGAAACGGCCGGTACAGGTGATCCCGGAGAAGGTGGAGGTCCTGTGGCGCCATGTCC GGTGTGACGGGGCGGAGGTCGAAACCCTCGAGCGGGAGGAGCGTGCGCGCCGGTTCGATCTGGGCCGCGG GCCGGCCATGCGGTTCCTGCTGCTCGAAACCGGGCAGCAGCGTTGGCGTTTCGTGATCACCGCCCACCAC ATCGTGATCGACGGCTGGTCGCTGTCGCTGCTGGTGTCCGAACTGCTGGCGCTGTACCGGGCCGCGGGGA ACACCTCCGCGCTGACCGACGCGCCGCGGCCCTACCGCGACTACATCGGCTGGCTGGCGGCGCGCGACCA GGACCACGCCCGGTCCCTGTGGCGCGCGCACCTCGCGGACCTCGACGGCCCCACGCTCCTGGCCCCGGCA CTGGGCGCCGCGACGATCGGCGACGGGCTGCCGGGCATGGTCGAGCTGACCGCCGACCCCTCCATCACCG AGACGGTCGTCGACGCGGCGAGGGCCCGCGGTGTGACCGTGAACTCGCTGGTCCAACTCGCGTGGGCGCT CACCCTGTCGTCACTGACCGACCGGGACGACATCACGTTCGGAGTGACGGTGTCCGGGCGCCCCGACGAC CTCGCGGGCGTCGAGTCGATGATCGGACTGTTCGTCAACACGGTCCCGTTGCGCGTCCGGCTCGATCCGG CGCTCACCGTCGGTGCGCAATGTCGTGCGCTGCAACAGGATGCCGCCACGCTGCGGGACCACAGCTATCT CGGCCACGCCGAGCTCAGGGCGCTGGCCGGGGTGGGCGAGTTGTTCGACACCCTGCTGGTGTACGAGAAC TTCCCGCCGGGCGCGGTGGCGGGCGCCGGCACGCTGGAAGCCGCCGGTGCCACGTTCACGCCGGTGGCGC TGCAGAGCCTCGCGCACTTCCCGGTGACCATCGCCGCACACCTCACCGACGGTCGGCTGACCGTGCTGGT CGAGGCGAAAGACGGTGCGCTCGGCCAGATCACGCCCCGGGCGCTGGGGACACGCCTGCTCGCCACGGTG TCACGCATCGTCGAGGGCTGGGACCGGCCGCTGCGCGAGATCAGCATCTCACCCGACGATCACCCCGTCC CCGTCCCCGTCCCCGTCTCCGAAGCCGCCCCGACCCCGTCGCCCAGCACCGGCGTGCACACCCGGTTCAC CGAAACCGCCTCGGCCAGGCTGGGTTCGGTGGCGCTGAGCTGGGACGGCGGTGCACTGACCTACCGTCAA CTCGACGAGGCCGCGGACCGGTTGGCCGCGGTGCTGACCGCCTCGGGAGTGCGCACGGAGACGCCGGTGG CGGTCCGGCTGCGGCGCGGCGCGGATTACGTGGTGGCGATGTTCGCCGTGCTCAAGGCCGGCGGTGCGAT CGTGCCCCTGGACCCGGCGATGCCGGCCGACCGCATCGAGGTGATCCTGCGGCGCGCAGGGGTGACCGTC GTCGTGGACGAGGACACGGTCGCCCGGGCCACCGCCGAACCGCCCGCGCAGTACCGTCCGGCCGAGACGC TGCCCGGGCAGGCCGCCTATGTCGTGTTCACGTCGGGAACCACCGGAGAACCCAAGGGCGTCATCGGAAC TCACCGGGCACTGCTGGCCTACGCCGACGACCACATCGGCGCGATCCTGCGGCCCGCGGCCGACCGGGTT GGCCGGCCTCTGCGCATCGCGCATGCGTGGTCGTTCACCTTCGACGCCGCGTGGCAGCCGCTGGCGGCGC TGCTGGACGGGCACTCCGTGCATATCGTCGACGACGCCACCCTACGCGACGCGGAGGCGCTCGTCGACAC CATCGCCCGGTTCGGTGTCGACATGATCGACACCACGCCCTCGATGTTCGCCCGGCTGCGCGACGTCGGG CTGCTGTCGACCGTTCCGCTGGCGGTGCTCGCACTCGGTGGTGAAGCCGTCGATCCCGGTCTGTGGCAAC AGATCCGGCAGGAGTGCGCACGCACGGGGATGTCGGCGTTCAACTGCTACGGCCCCACCGAGACGACGGT GGAGGCGGTGGTGGCCGAGATCGGCGCCCACGAGCGACCCACCATCGGCCGACCAACCGCGCCCACCCGC GCCTACGTGCTGGACTCGTGGCTGCGGCCGGTGCCTTCCGGGGCCGCCGGCGAACTGTATCTGGCCGGTG GCCAGCTGACCCGCGGATATCTCGGCCGGCCGGGGGAGACCGCGGCACGGTTCGTTCCCGACCCCTTCGT TCCCGGCGCGCGGATGTACCGCACCGGCGACGTCGTGCGTCGCGCCGTCGACGGGACATTGGCGTTCCTC GGCCGATCGGACACCCAGGTGAAGATCCGGGGCTTCCGGGTGGAGCCCGGCGAGGTCGCCGCGGTGCTGC GCACCCACCCGGCCGTCGGTCAGGCGTACGTGGCGGTGCGCCCCCACCGGGACGGACACCGGCTCACCGC CTACGTGACCGGCGAGCCGCAACTGCGTGAGCTCCGCGCATGGCTGGGCAGGAGGTTGCCCCGCTATCTG ATGCCGAACGCGATCGTCGCCGTCGACCGGATCCCGCTGACCGCCCACGGCAAGATCGACGAGCCCGCAC TGGTCGCACTCGAACCGGCCGCCGACGGCGCGGCGATGCGGCCCCAGACCCCGACCGAGGTCGAGCTGGC AGCCGTACTGGCCGAGGTTCTCGGGGTGGGCGAGATCGACGTGACGGCCGATTTCCTGGAGCTCGGTCTG GACAGCATCGTGGCGCTGTCGGTCGTGCAGACCGCGCGCCGTCGCGGTGTGGTGATGCGCGCGAAGCTGA TGCTGGACTGCACCACCGTGCGCGAGCTGGCCGCCGCGATCGACGACGCCGGCAGCGGGCCACCGTCGGC CGACGAGGGCAGCGGTCCGATCGCGTTGCTGCCCAACGCCCATTGGCTCTACGAGTTCGGCGATCCCCGC CGGCTGGCCTCGACGGAGGCGCTGCGGCTTCCGCGCGGTATCACCGGACCGCAGATCAGGGCGCTGCTGC AGAGTGTCGTCGACGCCCACCCGGTTCTGCGCACACGACTCGACCGGGCCTCCATGACGCTGGTCGACGG TGGCTTCGACCTTCCGCTGACCGAGATCGAGGTCGGCGACGAGGGGAGCGCGGCGGTGGCCCGTCACGCC GCGGCCGCGCTGGAACACCTCGACCCGCAGCGGGGGCCGCTGTGGGGCGCGGTGTGGCTTCGCGGGAGCG ACGACGCGGACTCGACGAACAGTGTCCTGCTGCTCACCGCGCACGTGCTGGCGATGGATCCGGTGTCATG GCAGATCGTCATCGGTGAGCTCGACGCGGGTTGGCACGCCCTCGCCGGCGGTCGCGCACCGGCGGCCCTC GGCGAGCACACCGGCTACCGGCAGTGGTCGAACCTGCTGCGCGAGCGGGCCGAGAGCTCGGACGGCTTGC AGTTCTGGCTGGACCAACTCGGCGGTCCCGACCCCGCGCTCGGGGCTCGGCGGGTGCGGCCCGACACCGA CCGCGCCGGGGACGTCCGTGTCACCGTCACGGTCACCGGTGCCGACGTCACGGCACGTCTGCTCGCGGGG CCGCACCCGGTGCTGGACGTGCTGGTCGCGGCCCTCGGCCGCACGATCACCGAATGGCGTGGACGCCGCA ATCAACCCACCCCGCCACCGCTGGTGGCGCTGGAGACACACGGACGCGCCGCCCTTGCCGAAGCGGACAC CGCCGACACCGCCGACACTTCGGACACGGTCGGGCTGCTCTCGGCGATCTACCCGCTCCGGCTGCACGGC GACAGCCCCGGTGCGACAACGGAACTGATCGCCGGGGTGCCGGCCGGGGGAATCGACTACGGACTGCTGC GGTACCTGCGCCCCGACACCTCCGAGGTGTTGAAGGCCCACTCGGAGCCGCAGATCCTGTTGAACTATCT CGGGCGCACCGACCTGGTTCCCTCCGGCGCGATCGTCGCCGATCGCCTGCTGCTGAGCGGCCTGTCCACG GTGCCCGAGCCGAACGCGGCGGTCCGCCACGAACTCACCGTGGTCGCCGCGGTCGTCGCCCACGGTGACG AGCCGGCGCTGGTCGCGCAGTGGCGAGTGCTGCCGGAGATCCTGAGCGACACCGACGTCGAGGAGATGCA GACCATCTGGCAGGACACACTGCGGGAGGTTCTGCGATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 2192924-2198212	Sequence	Mspyr1_21330 - amino acid adenylation enzyme/thioester reductase family protein
ORF.. 2198209-2202588	Sequence	Mspyr1_21340 - amino acid adenylation enzyme/thioester reductase family protein
ORF... 2202585-2203877	Sequence	Mspyr1_21350 - lysine/ornithine N-monooxygenase