Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 2192924
End position: 2198212
Length: 5289

DNA sequence
>NC_014814, from 2192924 to 2198212 (5289 bp); Mycobacterium sp. Spyr1 GTGACTGACACAGACCTGGACGCCCGGCGCCTGGAGCTGCTTCGCCGGCGACTCGCCGGATCGGGGCTGC GTGAGCAGGACCCCGCCGAGCCGGTGGCCGTCGCCGCGGACCCCGCACGGGGCGAGTTCACCGACGGGCA GAAGCGCATGTGGTTCGTGCACTCCGTCGACCCGAGCGGCGTCCTGCTCAACGTGTGCCTGTCCTATCGG ATCTCCGGCGAGGTGGATCTGGCCCGACTGCACGACGCCGTCACGGCCGTGGCCCGCCGGCACCCCGTGC TGCGCACGACTTTTGCCGCCGACGAGGCCGGAGAGCCGCAGCGTACGGTGCACGACGACCTGCTGCCGGG GTGGACGGTCCAGGACATCTCGGAGCTGTCCGAGCACGCGCGCCGGCTACGCCTGGAGGTGTTGGCGCAG CGGGAGTTCGGCACACCGTTCGACCTCGCGACCGAGTCGCCGCTGCGGATCACCGTGGCGCGCACCGCGC CCGCCGAGTACATCATGCTGCTCGTCGTCCACCACATCGCGTGGGACGACGGCTGCTGGCGGGTGTTCTT CGCCGATCTGACCCGCGCCTACACCGGCGCCGAACTCGGACCGACCCCCGCGCCGGTCGTAGAACCGCCC ACCTCGGACGAGGATCTGCGGTTCTGGCGCTCGGCGATGACCGATCTGCCCGAGCCACTGGAGCTTCCGG GGCCGCACGGGTCGGCGGTACCCACGACATGGCGCTGCCGACGTGTCACGGCGACACTGTCGGCCGACAC CGTGGAGCGTGTGCGCGTGGCCGCCCGCGAGGCCGGTGCGACGCCGTACATGGTGATGCTGGCCGCGTTC GGGGCGCTCCTGCACCGGTACACCCACGCCGACGACGTCCTGATCGCGAGCCCGGTGCTGCTGCGCGGGC CCGACAGCGAGGACGTGATCGGCTACCACGGCAACAGCGCGGTGATGCGACTGCGGCCGCAGCCGATGCA GACATTCACCGAACTGCTCTCCCGCACAAGAGACTTCGCACTCGACGCGTTCGCCCATCAGCAGGTGAAT CTCGACCGGGTGGTGCGCGAGCTCAACCTGGACCGCCGGCACGGCGCCGAGCGGATGACCCGGGTCAGCT TCGGCTTCCGCGAACCCGACGGCGGCGGGTTCAGCCCGCCCGGGGTGACGTGCCGGCGCGCGGAGCTGCG GGGCCAGATCACCCAGCTGCCGCTCGGGCTGATGGTGGAGCTGCACGAGTCCGGTGCCGTGGTGGAGGCC GAGTTTCTGGTCGAGGTGCTCGACGACACTCTCGTCACGCAGATGCTCGACCACTACGTGGTGCTGCTCG AAGCGGCTGTCGCACAACCGGACACGGTGCTCTCCAGGTTGGAGCTGATGGATGGCGACGACGCCGAATG GCTGCGCCGGGTATCGCATGGTCCCGAGTTCGACGTGCCGGCTGCCACCATCGGTGAGCTGATCGAGGCC CGCACCGACCTGACCCCCGACACCGTCGCGGTCGTGTACGAGGGCCGGCGGTACACCTACCGCGAGATCA ACGAATCGGCCAACCGGGTCGCGCACTGGCTGATCGGTCAGGGGATCGGTGCCGAGGACCGGGTCGCGGT GCTGATGGACCGCTCGCCCGAATTGCTGATCACCGCCCTCGGGATCATCAAGGCCGGCGCGGTCTATCTA CCGGTCGATCCGAGCTACCCGCAGGACCGGATGTCGTTCGTGCTCAGCGACTCCGACCCGACGCTGGTGA TCCGCGAGCCCGTCGCCGGGTTGGACGGCCATCGCACCGACAATCCCACCGACGACGTCCGGGTCCGGCC GCTGCGGCCGGAGAACGCGGCCTACCTGATCTACACCTCCGGTTCGACCGGACAGCCCAAGGGTGTGCCG GTGCCGCACCGTCCGGTCGCGGAGTACTTCGTCTGGTTCAAGGACGAATACGAGATCAGTGAATCCGACC GGCTGCTGCAGGTGGCCTCACCGAGTTTCGACGTGTCCATCGGCGAGATCTTCGGAACGCTGGCCTGCGG TGGGCGCCTGGTCATCCCGAAGCCGGACGGTCTCACCGACGTGGCCTATCTGACCGAACTGCTGCAGCGG GAGGGCATCACCGCGATGCACTTCGTGCCGTCGCTGCTCGGATTGTTCCTGTCCCTGCCCGGGGTGAACC AGTGGCGGACGCTGGAGCGCGTCCCGATCGGGGGTGAGGCGCTGCCCGGCGAGCTCGCCGACAGGTTCCA CGCCACCTTCGACGCGTTGCTGCACAACTTCTACGGTCCGACCGAGACGATCATCAACACCACCCGCTAC AAGGTCGAGGGCACCCAGGGATCCCGGATCGTGCCGATCGGCACGCCGAAGATCAACACCACGGTGCACC TGCTCGACGACGCGTTGCGACCGGTGCCGGTCGGCGCGATCGGCGAGATCTACATCGGCGGAGGCCACGT CGCGCGCGGATACCACCGCAGGCCGGCGCTGACCGCCGAGCGCTTCGTCGCCGATCCGTTCACCGTCGGT GGCCGCCTCTACCGTTCGGGTGATCTGGCCCGCCGCAACGCCGACGGCGATCTGGAGTTCGTCGGGCGGG CCGACGACCAGGTCAAGATCCGCGGCTTCCGTATCGAGCTCGGCGAGGTGGCCGCAGCGATCAGCGTCGA TCCGTCGGTCGGCCAGGCGGTCGTCCTCGGCCAGGATCTTCCGGGTCTGGGCATGAGCCTGGTCGGCTAC GTCACGCCGGCCGCGGGGGAGGACGTCGACCTGGCCAGGGTGCGCGAACGCGTCGCCGCCGCGCTGCCGG ATTACATGACACCGGCGGGCTACGTCGTCCTCGACGAGATCCCGATCACCGCCCACGGCAAGATCGACCG CGCCGCGCTGCCGCTGCCCGAGATCACCACCGCCACCGACTACCGCGAACCACAGACAGAGACCGAACAG CATCTGGCGCAACTGTTCTCCGAGCTGCTCGGGGTCGCGCGGGTCGGCGTCGACGACTCTTTCTTCGAGC TCGGCGGGCATTCGCTGCTGGCCACCAAGTTGGTCAGCGCGGTGCGCTCACGCTGCGGTGTGGACATGGA GATCCGGGAGATCTTCGAACTCGGCACCGTGGCCCGGCTCGCCGAACGTGTCGACGAACTCGCCGCCGGC GGGTCGGCGCCGCTGCGGCCCCGGCTGCTCGCCGTCCCGCACGACGGCCCGGCGCCGATGTCGGCCGCGC AGCTGCGGCAGTGGTTCCAGTACCGCATCGAGGGTCCGGGACCGGTCAACAACGTGCCGTTCGCGGCCCG GTTCGCCGGGCCGTGCGACGTCGACGCGCTGATCGAGGCGGTCCGCGACGTCGTCAACCGGCACGAGAGC CTGCGCACCACGTTCTGCGAGATCGACGGTGTGCCCTACCAGTTGGTCAACGCCCCGGATCCGGGCTTCC CGGTCCGGCGCGACAGCGGCCCCGACGATGCGTGGCTGGTCGGCGCGCTCGACGACGAACGCCGGCGTGT CTTCGACCTGGAGAACCAGTGGCCGATCCGGGTGGCCGTGCTGTCGATCCCCGGTGAACACGCGTTGTCC GTCGTGGTCCACCACATCGCCGCCGACCACTGGTCGGGTGGGGTGTTGTTCACCGAACTGCTCACCGCCT ACCGGGCCCGGCGCGCCGGGGAGGCGCCGACCTGGGCGCCGCTGCCGGTGCAGTACGCCGACTACGCGGC CTGGCAGTCGGCTCTGCTCGCGGACGACTCGGGAATCGTTGCGGCACAACGCCGGTACTGGACCCAGCAA CTCGAGGGGCTGAGTGCCGAGACCGGGCTGCAACCCGACCTCGTACGCCCGTCGGTGCCCAGCGGCGCCG CGGACGCCGTCGAGTTCACCCTCGACGCAGGGATCCGGGAGAAGCTGGTGACGTTGAGCCGGGAGGTCGG CGCCACCGAGTTCATGCTGCTGCAGGCCGCGGTCGCCGTGACGCTGTACAAAGCGGGCGAGGGCCCCGAC ATCCCGCTGGGCACACCGGTGGCCGGACGCACCGAGTCCGAACTCGACCAGCTGATCGGGTTCTTCATCA ACATCCTGGTGCTGCGCAACGATCTGCGCGACAACCCGTCGCTGCGTGACGTCGGGTTCCGGTCCCGCGA GATGGCCCTGGCCGCCTACGCCCACCAGGATCTGCCGTTCGATCAGGTGGTCGACGCCGTCAGCCCGGTG CGGTCGCTGTCGCGCAACCCGTTGTTCGGCGTGGTCGTCCATGTGCGAGACGAGCTTCCGCAGGACAGGG TCATCGCTACCGGTCCGGACGGCGAGACCACGTTCACCGCACTGGAACCGACCTTCGACATGGCCCACGC CGATCTGTCGGTCAACTTCTTCGCCGGCGAGAACGGTTACCGCGGGCACGTGATCTACCGCACGGAGCTC TACACGCGCGCCACCGCCGAGCGGTTCGCCGGATGGTTGAGCGCGGTGATCACCGCGTTCGCCGACGATC CGGAACGGCGCGTCGACGACGTGGTGATCGCGGAACCCGTGCGCCCCGGCGCCGGCGCCGAGGCCGTGCT GCTCGACGGTGCGGGCCATCCGGTGCCCGTGGGTGTGGTCGCCGACGTCCACTACACCGGTGCTCCGGAC CGTCGGGGTGATCGTGCCCGCTGGACCGAGGACGGCCGGTTGCAGTACGTCGAGCGCGTGGACCACGACC TGGAGGCCGCGCTGGCTGCGGTCCCCGGCGTCGCGGCGGCCGCGGTCCGCACCTGGGAGATCGGACACCG CACCGCGGTCGTCGGCTACGTGGTGCCCTCCGAACCCGTCGACGACCCGGCGTCGTTCGCCGAAACCGTC CGGGCGGCCGTGGCGCCGGAGTCTGCCCCGACCTCGATCACGGTCCTGGACGCGCTGCCCGCGACTCGGC GGGCGCTGCCCGCGCCTCGCGTCGGGGGGCCCAGCGAGCCGGCGCGCACCGACACCGAGAAGGCACTCGT CGGGATGCTCGCCGACGTTCTCGTCGAGCTGCACGACGGCACCGGCATCGGGCGCTTCGACGACTTCTTC GAACTGGGCGGCGACAGCATCCTCGCGGTGCAGCTGGCGGCGAGGGCCAAGCAGGCGGGATTGCGGCTGA GCGCCCGAATGGTGTTCGAACACCCCGTCGTTCACGACCTCGCGGTGGCGCTCGACACGGCGAAGACGCC GGAGGTCGCGGATGTGCGGCACGACCCGATGTCGGCCTCCGGGCTGACGCCCGAGGAACTGGCGATGGTC GCGACGTCCTGGGCGGCCGAACAGGACGGGTCGCCGTGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 2190045-2192927	Sequence	Mspyr1_21320 - acyltransferase family protein
ORF.. 2192924-2198212	Sequence	Mspyr1_21330 - amino acid adenylation enzyme/thioester reductase family protein
ORF... 2198209-2202588	Sequence	Mspyr1_21340 - amino acid adenylation enzyme/thioester reductase family protein