Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 2078259
End position: 2079308
Length: 1050

DNA sequence
>NC_014814, from 2078259 to 2079308 (1050 bp); Mycobacterium sp. Spyr1 CTACCGGCATCCATCCGACTCTCGGACACCCTGAGGCGAGGGCGATGTCACTTCGTCTGCGAGCTGTACG TCGACCGGCAGACCCGTCTCCAGTGATTCGACGCCCGCGGCGCACACCGCCGCGGCCGCGTAGCCGTCCC ACGCGGTGGGGCCGCACACGTTGGTCCCGCTGTGGACGGCATCGACCCAGCGCTGGATCTCGGTGTCGTA GGCCGGGCCGAAGCGTTCCCGGAATCCGGGGGTGAGGGTGCCGCCCCAGATGCCGGGTCCCTTCTTACGG ATCAGCCCGACGTCCAGGCCGATGATCGCGCTGCCGTGCTCGCCGATCACCTCCGTGCGGACCTCGTAGG CGACCCCGGTGGTCACGAACAGCTCGACGTCGACGTGGCGCCCCGACACGGTGCGCAGGATGGCGATCTG TGGATCGACGACGCCGTCCGGCGCGCCGGGGTTCGAGGTGGGTTTGATGATCTGTACGGACGCGATCTCC TCGCCGAACAGATATCTGGTGATGTCGACCTCGTGCGCCAGGGAGTCCTTGACGATCAGCGAGCTGTCGA AATAGGCCGGCACGCCCGGGTTGCGGTGCACGCAGTGCATCATCAGCGGATTGCCCAACTCGCCGCCGTC CAGCAGAGCTTTCAACCGCACGTACTCGTCGTCGAACCGTCGCATGAAGCCGACCTGGATCAGGGGCCGG CCCAGGTCGGCTTCGCGTCGGGCGATCTCCAGCGACGTCGAAACGTCTGTGGTGAGCGGCTTTTCGCACA TGACGGGCTTGCGCTGGTCGAGGCAGGCGAGCAGGTGCTCCTCGTGCGTGCCACCCGGGGTGGCCAGCAC CACGGCGTCGACGTCGGCGTCGGCGATCGCGTCCAGCGGGTCGGCGACCGCACTGCATCCCGGGATGCTC GCGGCCAGCTGTTCGGCCTTCTCGACGAGGTGGTCGTTGACCACCGCCACGCGGGCACCGGAGATGCGGG AGGTGATGCGTGCGACGTGGTCGGCGCCCATCACGCCGACGCCGAGCACGGCGATGCGGAGATCGGTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 2077861-2078292	Sequence	Mspyr1_20320 - hypothetical protein
ORF.. 2078259-2079308	Sequence	Mspyr1_20330 - dehydrogenase (Protein is coded in complementary strand; from 2079308 to 2078259)
ORF... 2079305-2081254	Sequence	Mspyr1_20340 - acetolactate synthase (Protein is coded in complementary strand; from 2081254 to 2079305)