Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 1674226
End position: 1676055
Length: 1830

DNA sequence
>NC_014814, from 1674226 to 1676055 (1830 bp); Mycobacterium sp. Spyr1 ATGTTTGACACGGTGACGTCGCGCGCTGCCGGTACCACCAACCCGTGGCCGGCGCTGGGGGCACTGCTGA TCGGGTTCTTCATGATCCTGGTCGACGCGACGATCGTCGCGGTCGCCAATCCGGCGATCATGGCGAGCCT GAACGCCGGATACGACGCCGTGATCTGGGTGACGAGCGCCTACCTGCTCGCGTATGCGGTTCCGCTGCTG GTGTCGGGTCGCCTCGGCGACCGGTACGGGCCCAAGAACGTGTACATGGTCGGCCTGACGGTGTTCACCG TGGCATCGCTGTGGTGTGGCCTGTCCGATTCGATCGGGATGCTGATCGCGGCCCGCGTCGTGCAGGGTGT CGGCGCGGCGCTGCTGACCCCGCAGACGATGACGGTGATCACCCGGACGTTCCCCGCGCACCGCCGCGGC GTGGCGATGAGCGTCTGGGGTGCGACGGCCGGGGTGGCGACGCTGGTGGGCCCGCTGGCCGGCGGTGTCC TCGTCGACAGCCTCGGCTGGCAGTGGATCTTCATCGTCAACGTCCCGGTCGGCATCGTCGGGCTGGTGAT GGCCCTTCGGCTGGTGCCGGCGCTGCCGAACCCGGTCCGCCAGGGGTTCGACGTGCCCGGCGTCGTGCTG TCGGGTGTGGGGCTGTTCTTGATCGTGTTCGCCCTGCAGGAGGGGCAGAGCCACGGCTGGGCGCTGTGGA TCTGGGCGACGGTGCTGGCGGGCGTCGCGGTGATGGCCGCGTTCGTGTACTGGCAGTCGATCAACACCGC CGGCGCCCTCATCCCGCTGGTGATCTTCCGGGACCGGAACTTCTCGATGTCGAATCTCGGTGTGGCGACC ATCGGGTTCGTCGCGACGGGCATGATCCTGCCGCTGATGTTCTTCGCGCAGGCGGTCTGCGGGCTCACCC CGACGCGGGCGGCCCTGCTGACGGCGCCGATGGCGGTGGCGACCGGGGTGCTCGCGCCCTTCGTGGGCAA GCTGGTCGACCGCGCGCATCCGCGATCGGTGGTCGGCTTCGGTTTCTCACTGATGGCGATCGGCGTGACG TGGCTGTCGATCGAGATGACGCCGACCACACCGATCTGGCGACTGCTGCTGCCGCTGACCGCGATGGGCG CGGCGATGGCGTTCATCTGGTCCCCGCTGGCGGCGACCGCGACGCGCAACCTGCCGCCGCACCTGGCCGG GGCGGGGTCGGGCGTCTACAACACCACCCGCCAGGTGGGCTCGGTACTCGGCAGCGCGGGGATGGCCGCG CTGATGTCCTCGGAACTCTCGACCAAGATCCCGGGCGCCGACGCGGTGCCGCAGTCCGAGGGCGAGGTGG CCGCCCTCCCGCAGTTCCTGCGCGAACCGTTCGCCCTGGCGATGTCGCAGTCGATGTTGCTGCCCGCGTT CGTCGCGCTATTCGGGGTCATCGCGGCGCTGTTCCTGTTCGACCTGCTCGGCGACCGCGACGCCGCCGCA CCGCCCGCGCCGGACGTGGAACCGCTCGAGCCGTTCGACCCCGACCACCCGCTGGACTGGGCCGACGGTG AGGACGACTACGTCGAGTACGAGGTGTCGCGCGAGGAGTTCGACAGGCTCGCCACCGACGGCACGGCGGA CGGGGCGGCCTACAACATCACTGAAGACTTCACTGAAGACATCACCGACGTGCTCGACACCGTTGTCGAG GCGCCGCCCGCACCTGCCGACTCGACGCCGTCTCAGCAGTGGCGCGACCTCGTCGACCAACTCCTCGACG TCTCGTCGCCACCGGGCGCTCCGCGCAACGGCGTCGGATCGGCCGATGCGGAGCATCCCGGCGGCGGTCC GCGCGTCTAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 1674226-1676055	Sequence	Mspyr1_16530 - EmrB/QacA subfamily drug resistance transporter
ORF... 1676052-1677455	Sequence	Mspyr1_16540 - hypothetical protein (Protein is coded in complementary strand; from 1677455 to 1676052)