Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 1545309
End position: 1546775
Length: 1467

DNA sequence
>NC_014814, from 1545309 to 1546775 (1467 bp); Mycobacterium sp. Spyr1 ATGGACGACGACGGCCTCAAACCAGGGTGGTGGACGCTGATCCTGGTCGCGGTGTTCGCGGTGGCGATCT TCGTGACCTACGCGCTGTTCACCGGTTCGCTGAAATCCACTGTGCCGGTGACGCTGACCTCCGACCGCGC CGGCCTGGTGATGGAGACCAACGCCAAGGTCAAGCTGCGCGGTGTGCAGGTCGGCCGCGTGTCCGACATC TCGGTCACCGGCCGTCAGGACCCCACCGTCGCGCTGCAACTCGAGATCGATCCCGACCAGGTGCAGTACA TCCCGGCCAACGTCGAGGCGCAGATCCGGGCCACCACCATCTTCGGCGCCAAGTTCGTCGACCTCGTCTT CCCGGACGACCCGAGCAACCAACGCCTCGAAGCCGGCCAGGTGCTGCAGTCACGCAACGTGACGACCGAG GTCAACACGGTCTTCCAGAACCTCGTCGCGGTCCTCGATCAGGTCGATGCCGCGAAGCTCAACAGCACCC TGTCCGCACTCGCAGACGGTGTCCGCGGCCAGGGTGAGCGCATCGGCCGGGCCACCACGGACGCCAATCA GGTTCTGCTGGCCCTCAACCCGCGCAACGAGACCATCACCGAGGACCTGCGCGCACTCGACCAGTTCAAC CAGACCTACAGCGCCGCCGCCCAGAACATCCTGGCGACGCTCGACGCCGCGTCGACCACCAGCACCACCG TGGTCAACCAGGCCAAGGAACTCGACGCGCTGCTGCTCGCGGCGATCGGACTGTCCAACAGCGGGATCGA CCTGCTGGCGCCCAATCAGGCCAACCTGATCAAGGCGGTCAACACACTGGCGCCGACGACCAACCTGCTC CACAAGTACAGCCCGTCCTACACCTGCCTGCTGCTGGGCGCCAAGCACCTTCTCGACGAGGGCGGCTACG AGGCGCCCGGCGGCAACGGACGCACCCTGGTCCTCGACACCAGCCTGTCGTTCGGCGACGACCCGTACCA GTACCCGCGGCATCTGCCGATCATCGGGGCCAAGGGTGGGCCCGGCGGCAAGCCCGGCTGCGGTTCGCTG CCGAAGGTCGCCGAGAACTGGCCCGTGCGGAACCTGGTGACCAACACCGGGTTCGGCAGCGGTCTGGACT GGCGTCCGAACCCGGGCATCGCCTTCCCCACCTACGCCAACTACCTGCCGGTCACCCGTGCGGTACCGGA GCCGCCCAGCGTGCGGAACCTGTTCGGCGGTCCCGCCATCGGACCAATTCCGTATCCGGGCGCCCCGGCC TACGGTGCGCAGCTGTACGCGCCCGACGGCACGCCGTTGTGGCCGGGCCTGCCGCCCGCCCCGCCGCCCG GCGCGCCGCGCGACCCCGGTCCCCGGCCCGGATCGGAGCCGTTCGTGGTTCCGCACCCGTCCCAGATGCA GCCGACCGCCGTGCCGTACCCGTTCGTGCCGCCACCGGTCCCGGCCCAGCCCGGACCGCCCCGATAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 1545309-1546775	Sequence	Mspyr1_15270 - virulence factor Mce family protein