Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 1397429
End position: 1397608
Length: 180

DNA sequence
>NC_014814, from 1397429 to 1397608 (180 bp); Mycobacterium sp. Spyr1 TCAGTCGTCACTGCGCGCTTCGGCACCCGACTCGCCGGCGTCGAAGTCGTCGTCCGAGCTCGAACGCCCC ACGTACGGTCCGTCGGACGGGTCGTCGTCGCCTTCGGATGCCCGTGAACCGGCGACCTTGGGGTCGGCGT CGGTATCGGCTTCGGCCTTTTCTTCGCGGGGGCTGCTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 1397429-1397608	Sequence	Mspyr1_13720 - hypothetical protein (Protein is coded in complementary strand; from 1397608 to 1397429)