Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 12231
End position: 13175
Length: 945

DNA sequence
>NC_014814, from 12230 to 13174 (945 bp); Mycobacterium sp. Spyr1 GTGAGCTCTCCGAAAGAGCCCGGGCCCGCCCATGCGGGTGACGGTGCGGCCCCTGCCGAAGGTCCCGGGA ACGGATCAGCGGCTCCCGGTCACGACGCCGGCGCGCGGCCTGCGCACGTCGCCGACACGACCGAGTTGCC GCCGTGGCAGCGGGGCCCGGCCACCCGGGCCAGGCAGCAGTCCCAGGCTCCGGAAGCACCGCGTCCCGAC GGTCCGCGCGGAGGCAATCAGGGCGGGCTCGACGCCCGGTTGAACCGGTTCATGGCAGGGGGATCCGCCC CGTCGGGTGCACCGGACGCTCCCCCGCGCAACGAGCCACCTCGCAATGACCCACCGCGCAACGAGCCGGC CCCGCGGAACGACCGCACCGACGTCGTCCGCCCCGAGCCGAAGCCGCGGCCCGAGGGCGGCCGGCCCGAG GGCGCCGCGTACGCCAGCGAACTGCCGGACCTGTCGGGCCCGCGGCCGCCGCAGCCGCCGCGCAAGCCCG TCGAGCGACCGTCGACGGAGTCGAAGCCGCCGGCCAAGGGTTCGCCGTCACCGGCGGGGCGGGTGCAGGT GGCGTCGCGGCAGCACCGGGGTCCGGTGCGCGCGAGCATGCAGATCCGTCGCGTGGATCCGTGGAGCACG TTGAAGGTCTCGCTGCTGCTGTCGGTGGTGCTGTTCTTCGTGTGGATGATCGCGGTGGCGTTCCTGTACC TCGTGCTCGGCGGCATGGGCGTGTGGAGCAAGCTCAACAGCAACGTGGGCGATCTGTTGACCAGCGCCAG CGGAAGCTCCGGCGGGGAGTTGGTGTCCAGCGGCACCATCTTCGGCGGGGCGGCACTGATCGGCCTGGTG AACATCGTCCTGCTGACGGCCGGAGCGACGATCGGCGCGTTCATCTACAACCTCACCACCGACCTGGTGG GCGGTGTGGAGGTCACGCTGGCCGATCGCGACTGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 12231-13175	Sequence	Mspyr1_00080 - hypothetical protein