Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 1059653
End position: 1061602
Length: 1950

DNA sequence
>NC_014814, from 1059653 to 1061602 (1950 bp); Mycobacterium sp. Spyr1 TCACTCGCAGCTGGTGTCGGCGGCGTTGGTCACGGTCAGATCCTCGGGCAGTTCGGTGGCCGGGGTCTTC GTGCTGCGGATGATGTGGACCTGAACCGACGAGCCGCTCGGTGACGGTGCGCCGACGGAGTTGAAATCGC TACCCAGGACGACCTGCACCACGTCGCCGAGGCCGCTCGCCCGTTCCACCGTCGCATCGCCGAAGGAGGA CGCGACCGTCGCCGCGGCTTCCTCGTTGCCCGGCGAGAAGAACACGGTCGTCCTCTCCAGCGGACCCGGG TAATCGTCCGGACTCTCCACGTTGAAGCCGTGGGACTGCAGCGCGGTGCTGGCGGTCGCGCCCAGTCCCG ACTGGCCGGTGGAGTTCGACACCTGGACGGTCACGTTCTGCGGATCGGTGGTGATGGCGTCGACGATCTC GTCGGACGGCCCTTCGCCGTCCACGCCGCTGCCCGGCGCGGCCGCCAATGACTCGGGGGTACCCGGCACC GGTGAGTTGTCGGGGTTGCGCTCCTCGGGAAGCGGGTCGTCGTTGATGATGGCGTCGAACAACGCGCGCA TGTCGTCGGTGCGGGGGATCTCATTGCCGTACTCGTCGGCGTATCCGACGGTCGGCACCGTGATGAACGT GATGCGCCCGGCGGTCACGCCCTGGACCGACTGGCCGAGATCGACGAGATCCTTGGTGTCGACGTTGTCG ACGTAGCTGTCCCCGATGAACATGTTCACCACGTTGTTGAGCTTGGACAGCGAGAAGAACGTCTCCTTGG AGATCAGCGAGCGCAGCAGCGACGACAGGAACAGCTGCTGCCGTTTGATGCGGCCGTAGTCGCCGTTGGT CTCGGTGGTGACCTGCCGGGCCCGCACGTAGTTCAGTGCGGTGTGTCCGTTGACGACCTGTCGCCCGGCG GTGGGCAGGACCGTTCCGAGCTCGTAGTCCTCGATCGGGGTGGTACTGCACACCTCGACGCCGCCGAGCG CGTCGACCATCTTGGAGAAGCCGACGAAGTCGACCGCCATGAACCGGTTGACGTGCAGGCCGGACAGCTT CTGGATGACCTTGACCAGACACTTGGGGCCGCCGACGGCGAACGCCGAGTTCAGCTTGTACTCGGTGTAG ACCTCTTCTTTGCCGTACATCGGCGATTCCGGGTCCATGATCGGGCCGTACTCGCGGGTCTCGGGATCCC ACGGCTCGCACTTCATCGGCTCGATCTCGAGGTCACGCGGAAACGACACGGCCACAACGCGTTTGCGGTT GGCCGGGATGTTCACCAGCATCACGGTGTCCGACCGCGCGCCCGCCGCGTCCTCGGTGGTGCCCGCCCCG ATCTCGGCGTTGCTCCCGAGCCTGCTGTCGGTGCCGACGATCAGGAAGTTCTCGTCGCCGAACTGGGCGT TGGGGTCGACGATGTCGCGGGAGTCGGGGTCGAGCGCCGAGACCCGGTTGAGGAGATTGTTCTTCGCCGA CTGCCACTGCCAGGCGCCGCCGGTGAGCACCAGCGCGAGCACTGCGACCAGCGCCGCCGCGACGCGGCCG GCCATGACGGTCCGCCGGTGTGAGCGCACCGGCTTGGCCACGACGTGCTGCGGCGGCCGGGGCGCCTCCA GGTCCGGAAGTTCGGATGCCCGCGGATCGATGACGGGCAGGACGGTCGTGTCGAACTCACCGGTGTCCGG ATAGGGCTCGGGTTCGGGTTCGGGTTCTGCGCGGTGCCGCTTCGGTTCGGGCGGCGGAGCACCGCCGTTG ATCTTGGCGATCAGGTCGGCGACGGTGACGGGCGCGGCCGGACCTTCGTCGGGCCCCGAGGCGTCGCGGC CGGCGTTGCTGTCGATGTCGCGTTCCCACGGCGCGACGTCGGGCGCGGCCCGGCGGGACCGCCGGATCCT CCCGCCGTCCCCGCGTTCGGCATGCGGACGGCCGGGAGTGGCGCTATCGCCGTCACTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 1059653-1061602	Sequence	Mspyr1_10430 - cell envelope-related transcriptional attenuator (Protein is coded in complementary strand; from 1061602 to 1059653)