Sequence retrieval

Genome: Thermoanaerobacter sp. X513
Start position: 47133
End position: 50427
Length: 3295

DNA sequence
>NC_014538, from 47132 to 50426 (3295 bp); Thermoanaerobacter sp. X513 TATCCGACATCTGACTTCCGACATCTGACTTCTGTTTGGGGTAGGGGAGCGTACTGTACGGGTAGAAGGC CAAGCGGAAGCGAGGCTGGACTGTACAGTAGAGAGAATGCCGGCATGAGTAACGAGAGTAAGGTGAGAAA CCTTACCGCCGGAAGCCTGAGGTTTCCTGGGGAAGGGTAATCCACCCAGGGTAAGTCGGGGCCTAAGCCG AGGCTTAAAGCGTAGGCGATGGGGAACCGGTAGAGAATCCGGTACCACCGAGGGCCGCAAAGAGATGCGG GGACGCAGCGAGATAGGTGGAGCGTGCGGTTGGTAGGGCACGTCCAATCAGGCACCGAAGGGTACGTAGG CAAATCCGCGTACTGGCAGGGGTCTGAGAAGGGGAGCCGGAAGGCGAAGCCACCGAAGCAGCTGCCGAGA AAAGCCGCTATCGAGGCCTGAGGTGCCCGTACTACAAACCGACACAGGTAGGCGAGGAGAGAATCCACAG GCGAGCGGGAGAACCCTCATTAAGGAACTCGGCAAAATGACTCCGTAACTTCGGGAGAAGGAGTGCCGAA AGGCCGCAGAGAAGAGGCCCAAGCGACTGTTTACCAAAAACACAGGTCTCTGCTAAGTCGGAAGACGAAG TATAGGGGCTGACGCCTGCCCGGTGCTGGAAGGTTAAGGGGAAGTGTGAGAGCACTGAACCGAAGCCCCA GTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCA CGAAAGGCGTAACGACTTGGGCGCTGTCTTGATGAGGGGCCCGGTGAAATTGTAGTACTCGTGAAGATGC GAGTTACCCGCGATTGGACAGAAAGACCCCGTGGAGCTTTACTGTAGCCTGTCACTGGATTTTGGTAATT CTTGTACAGGATAGGTGGGAGACAGAGAAGGTAGGGCGCCAGCCTTACTGGAGTCGACGTTGGGATACCA CCCTGGGATTACCGGAATTCTAACCTATAGCCGTGAAACCGGCGTAGGGACAATGGCAGGTGGGCAGTTT GACTGGGGCGGTCGCCTCCTAAAGGGTAACGGAGGCGCCCAAAGGTTACCTCAGCGCGGACGGGAATCGC GCGAAAGAGTGCAAAGGCAGAAGGTAGCCTGACTGCGAGAGAGACATCTCGAGCAGGGACGAAAGTCGGG CTTAGTGATCCGGCGGCAGTGAGTGGGAACGCCGTCGCTCAACGGATAAAAGCTACCCCGGGGATAACAG GCTGATCTCCCCCAAGAGTCCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGG GGCTGAAGTAGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTC GTGAGACAGTTCGGTCCCTATCCGTCGCGGGCGTAGGAAACTTGAGAGGCACTGCCCCTAGTACGAGAGG ACCGGGGTGGACGAACCGATGGTGTACCAGTTGCACCGCCAGGTGCACAGCTGGGTAGCCAAGTTCGGAA GGGATAAACGCTGAAAGCATCTAAGCGTGAAGCCCGCCTCAAGATTAGGTTTCCCATCCGAGAGGGGTAA GACACCTCGAAGACTACGAGGTAGATAGGCCGGGGGTGTAAGAGTGGAAACACTCTTAGCTGACCGGTAC TAATCAGTCGAGGACTTGACCGATAAGGCTTTGTGCAGTTTTGAGGGTCTAGATTTCCGGTGGCGATAGC GGAGGGGAAACACCCGTTCCCATTCCGAACACGGTCCACAAGGGGCCCCCAGACGGTCGCAAGACCGGCT GGGGTGCTAGTTAAGCCCTCCAGCGCCGATGGTACTGCTTTCGCGGGAGAGTAGGTCGCTGCCGGAATTA AAATATTCCTCAGTAGCTCAATGGTGGAGCAACCGGCTGTTAACCGGTAGGCTGTAGGTTCGAGTCCTAC CTGAGGAGCCAACTTTTAAGAGATGCGGATGTGGCTCAGCGGTAGAGCATCGGCTTCCCAAGCCGAGGAT CGCGGGTTCGAATCCCGTCATCCGCTCCAGTGAAATGAAAGGTTCTGAGCATTTCATAAAGTGCCAGAAC CTTTTTTTAGCGCCATTTTGGTGCCCAATGCAATTTAATTTTTTAATAAAGTATTGACATATTTTTATTT TTTTATAAAATACAAAACGAAGTTTATCAATTTTAGAAGGTGAAAATGATGCTAAATGGCAAAGTAGCAA TTGTAACAGGCGGCGCAGGAGATATAGGTAGAGAAATTTGTATAGAGCTTGCAAAAGAAGGAGCTTCTAT AGCAATACATTATAATAACAGTTATGAGCAAGCAGTAAAACTAAAAGAGTATATAAAAAGTAATTTTAGT TATGCGGAAATTTTTAAAGCAGATGTTTCGGACAGACGACAAGTAGACGATATGATCGATAATATTTACA ATAAATTTGGTCGCATTGACTATCTTATAAATAATGCAGGAATTGCCCAAATCAAACCATTTATAGAAAT AACAGAAGAAGATTGGGATAGGATGATGAATGTCAATTTAAAAGGTCTTTTCAATTGCACACAAAGTGTT TTGAGGTATATGTTGCCTCAAAAACATGGGAGTATAATAAACATTTCTTCAATTTGGGGTATTTCCGGTG CTTCCTGTGAAGTACATTATTCGGCATCTAAAGGAGGAATAATAGCTTTTACAAAGTCTTTGGCAAAAGA GTTAGGGCCTTCTAAGATAAGAGTTAATTGTATTGCTCCTGGAGTAATTGATACTAAGATGAATGACATT TTAAATGCCGAAGATAAAAATCATTTAATTGAGGATATACCACTTATGAGCATAGGGAAACCCCAAGATG TAGCAAATGCTGTATTGTTTTTATTATCTGACAAAGCAAACTTCATAACAGGGCAAGTCATAACAGTAGA CGGAGGATTTATTTGAAAGGCTTTGGTGAAAGTCTTATTTTTTATATCGACTTTTTCAGGAAAGATAGCT CTTCTTCAGACAATTCTCTCCACTCCCCAGGTTTTAGATTTTCGTCAAGGGTGAGATTACCCATTGCCAA TCTTTTAAGGTATATGACCTTTTTACCGACTGATTCAAACATTCTTTTTATTTGGTGGTATTTTCCTTCT CTTATATAAACGTAAACTTTCGACACATCAGAAGAAGAAAGAATTTCGAGGTTTGCGGGAAGAGTTTTAT ACCCATCTTCAAGTACAATGCCTTCATTGAAAGCTTTAATGTCGCTTTCATTGACAAAGCCTAATATTTT AGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF... 50114-50836	Sequence	Thet_0044 - pseudouridine synthase (Protein is coded in complementary strand; from 50836 to 50114)
45540-48835	Sequence	Thet_R0008 23S ribosomal RNA