Sequence retrieval

Genome: Spirochaeta thermophila DSM 6192
Start position: 618536
End position: 619927
Length: 1392

DNA sequence
>NC_014484, from 618536 to 619927 (1392 bp); Spirochaeta thermophila DSM 6192 TCACCCCGGGAGGCGTATGGTGAAGGTGGTGCCGTGCCCGGGGGTGCTGTGCACGTCGATGGTACCTTTG TGTGCCTGGACGATGTGCTTGACGATGGAGAGGCCGAGGCCGGTGCCGCCGGTGGCGCGCGATCTGGAGG TGTCCACCCGGTAGAATCGTTCGAAGATGCGGGGGAGGGCCTCGGGTGGGATGCCGGGGCCGCGGTCCTC CACCTGGATGAGGACGGATGAGCCGGTGCTTCTCCACCGTACCACCACGGGGGTGCCGGGTGGGGTGTGG GTGAGGGCGTTGTGGATGAGATTGTAGAGGGCCTGGACCATGAGGCCGGGGTTGAGGGGGAGCGCGAGGT CCTCCGGGCCTTCGAGGGTGAGGGGATGGGTCTCGCCTCTGGCTTGCCGTACGAGCTCCACGGCCCTCCG GATGAGTGGGGCGAGGGGTGCGCGTTCGAGGGGTACGGGGGCCTCCGCCTGCTCGATGCGGGAGAGGAGC AGGAGGTCTTCGATGATCTGGTGGAGGCGGTGGGTGTGTGAGAGGGCGGTCTCGAGGAAGCGCGTGCGGG TGGCCTCGTCGTCCGGGGGGAGGTCTTTGAGGGTCTCGAGGTAGCCGAGGACGGTGGTGATGGGGGTCTT GAGTTCGTGGGAGACGCTGGAGACGAAGTCGCGGCGGATCTGCTCGAGCCGTTCGAGGCGGGTGATGTCG CTCGCCACGAGGAGGAGGCGCTCCGAGGGGGAGGGGGGGAGGGGGATGAGGTGGAGTCGGATGGTTCTGG AGGAGGGGGGTGGGGTGGTGAGGGTGTGGGTGAGGGGGGTGCCTGAGGAGAGGGTCCTGTGGATGTGCTC GAGGAGGGAGGTGGAGCGGAGGAATTGGGGGAGGGGTTTCAGGCGGTGCTCGGGGGGGAGGGAGAGGATC TCGAGGGCGCGTGGGTTGGCGTAGGTGATGCGGAGGCGGCCGTCCGTGACCACGATCCCCTCTTCCATGT GGGTGAGCACGGTGTGGTAGAGGTGGCTCTCGGCGGTGAGGTCTTGGAGGCGGGTGTGGGTCTGGCCGAG GGTGTGGAGCAGGGCCTCCTCGAGGGGCTGAAGGGCCCGGGAGAAGGGGTTGGTGAGGGTTCTGGTGTGG GGGAGGAGGGGGGGAGGGGTGGTGAGGAGGCGGCGCTTGAGGGTGCGGAGGGTGCGGCGGAGGTGGAGGG CGTGGTGGAGGAGGAGGGAGGCGATGAGGAGGGTCTGGAGGAGGAGGGCGAGGAGGGGTGGGGTGAGGGG AAGCGCGAGGGGGAGGAGCGAGAGGGCGAGGATGGGGAGGAGGGGGAGGAGGAGGCGGACCGGCCAGAGC GGGTCAGACCGGCTGGAGGCGGTAGCCGACTCCCCGGACGGTCTGGATGTAGTCTCGTGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 618536-619927	Sequence	STHERM_c05360 phoR phosphate regulon sensor protein PhoR (Protein is coded in complementary strand; from 619927 to 618536)
ORF... 619869-620549	Sequence	STHERM_c05370 - transcriptional regulatory protein (Protein is coded in complementary strand; from 620549 to 619869)