DNA sequence >NC_014410, from 2198315 to 2198414 (100 bp); Thermoanaerobacterium thermosaccharolyticum DSM 571 TGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTT Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI