DNA sequence >NC_014410, from 2198307 to 2198406 (100 bp); Thermoanaerobacterium thermosaccharolyticum DSM 571 TAATTTTCTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTT Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI