Sequence retrieval

Genome: Herbaspirillum seropedicae SmR1
Start position: 4831597
End position: 4832325
Length: 729

DNA sequence
>NC_014323, from 4831597 to 4832325 (729 bp); Herbaspirillum seropedicae SmR1 TCATCGCGCGACCTGCCGCTGCAAGGCATCCACCAGCTCTGCATGGCGCACGTGGGCGCGTTGCGCCTCG GCCTGTTGCGCCGCCAGCAGGGCCTGGCCGTTGTGACTGATGGCCTCGATCAGGCGCTGCTGCTGCGCCT GCTCCTGCTGCACCTGGCGCTGCACCGCCTGCGCGGCCAAGGCGCCCAAGGCGGCGGCGCTCCAGGCCAG CCTGGCCTGCTCATGCGCGCTGGCCGCCCACCAGAGCCTGAGGATCAGATAACTGAGAATGCCCCAGGCG GCAATGCGGAACTTGGCCCCCAGCGTGTCGAGCACGGCGGCCAGCGCCGTCGGCGCCTGGGCGCCATCGA GTGCGCCGGAAGCTTCGCCCAGCGCCAGCCCCAGGCCGATGAAGGTCCCCAGCAGGCCGGCCATGAGCAA CAGCCCAGGCACCAGCCGCGCCCAGCGTTCGGGGCCGGTGGCCACGATCTGGCTCAGTTGCTCGGGCGTG ATCGATGAGGGCAGGCTTTGCGGGTCCGCGCTCAGGGCGGCCCATCGCTGTTGCCACTGCGCCGGTGTCG CTGCGCGGCGCACGAGCCAGGGCATGAGGATCAGCGCCAGCACGAACAGCAGCGCAAACACCAGTTGCAG CCCGCCCTCTCCTGCGGCTTGCCATTGCGGCAGCAGGAAACCCAGTACCGATCTATCCATGACGTGCTCC CGCCTCCATTCCGATCTGCAACATCTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 4831597-4832325	Sequence	Hsero_4224 - hypothetical protein (Protein is coded in complementary strand; from 4832325 to 4831597)