Sequence retrieval

Genome: Geobacillus sp. Y412MC61
Start position: 512529
End position: 515234
Length: 2706

DNA sequence
>NC_013411, from 512529 to 515234 (2706 bp); Geobacillus sp. Y412MC61 TTATGGACGGGTGCCAAGACCCGCTCCGGATCCGTTTTTTAACGGCGGGGAGACAGGCGGCGTTCCGCTG TTTGAGCCGGTGCCGCCGTTCGTACCATTCGTTTGGCCGCTGTTGTTTGTTCCTTGGTTGTTTGTTCCTT GGTTGTTTGTTCCATTCGTTTGGCCATTGTTTGGGTTGGCCCCGCCGCTGTTGCCATTTTCTTGTCCGCC GCTTGCGCCGCCGTTTTGTTCATTGTTGTTCCCCTGACCATTTTCGTTTCCGTTATTCCCGCTGTTGCCT TGATCGGTGCCGCCGTTTTGCCCGCCGTTGCCTTGGCTGTCATCCCCCGGTTGTTGTGGCGCGGGAGTTT GATCTTCTCCGCCGGGGACATGAACGCTGGCTATGGCCGGTTTGCTCATTCGTTCGCCGTCTTTTGCGTA CACGGCGATTGTGTACGTCCATCCTGGCGTCGGATTGGAAATCGTGATCGAACGGTCTTTTGTGGTGATC GTATTCGTTTGACCTTGGCCATCTTTGACGCGCACCTCGAAGATCATCTCATCGGTTTGCTCATCGTATG ACCACGAAACCGAGATGGCGTTCGCCGCTTGGTCGTACACAGCGGTCAACCCTTTCACCGCCGGCAGTTC TTCCGGCACGTCGTTCGCCGTCTCCGTCGGCTCAGTGCCGCGCACGAACAATTCATACGTAATTTCGCTT TCCGGCGTGTATTTGCCGGCGAGCTTCGGCGGGTTCGTGCCTTTTTTGATCGGCAGCTTGACGACGCTGT CCGGCTGCTTGAATTCCCCACTGCCGTCATCGATATGGGACATTAAGTTTTTCAACAAGTATTTCGGGAT TTTTTGTTCCTCTATACTAAGCTTTGCCGTGCTGCTTCGCTTGCTGAAGCCGACCCAAATGGCCGCCGTA TAGTTTGGCGTATAGCCGACAATCCAGCTGTCCGGCACATCGTCTCTTTGGAGGCCAAATTTGGCGCCGT CTTCGCCGTAGTTTGTCGTTCCTGTTTTGCCCGCGAGCTCAAGCCCTGGGACGTTAGCCAACACGCCGGT TCCGGAGCGGATGACCGTTTTCAGCACATCGGTGATCATATAAGCGGTATAGTCGTGCATCACCCGTTTC GGCTTTGGCTGCAAATCCATTTCCGTGCCGTCTGGGAAGACGATTTTCGTCACGGCATACGGCTTTGTGT AAATCCCGTTGTTGCCAAAGGCGGTGTAGGCCCCGGCCATTTGCAACGGTGACACATAGTTGGATACTCC TCCGATCGCATATGGCTCATATACTTCACTAAAGCCCATCCCAAGACGATTGGCAAACTCCATCGCCCGT TCTTTGCCGACAGCTTGGAACGTTTTTAACGCCGGGATGTTGCGTGATTTTGCCAGTGCCGTCCGCAAGG TGATCGGCCCCATATATCCCCCGCCGGCGTTGCGGATCGGCGTGCCGTTTGAATACGTGTACGGCTCGTC AACAAGAATGTGCGCTGTTGACCATTTTAAATATTCGATCGCTGGACCGTAATCCAAAATCGGTTTGATC GTCGAACCGGGCTGGCCGACCGGTTTGATGGCGTAGTTGAAGCCAAATTCGACGTGGTCGCGGTGGCGAC CGCCGCCTAACGCGCGAATGGCGCCTGTTTTTGTGTCGACGACGGCGATGCCGGATTGCAAATCCTTCTT ATTGGTAAAGTATTTGTCAGAGTTTAATAGCTTTTCTACGTATGTTTGAACGTCTTGGTCGAGCGTTGTA TAAATTTTCAGCCCATCTTCAAAGACGTTGACGTTCGCTTTGTCGGTCACTTCTTTGATCACTTCGTCAA TGAAGGCGTCATACGGCACGGAGCTTTGCGGTTTTTTCCGCTCAGCGAGCATCGACTGGATCGGCACTTG TTTCGCTTTTTCCGCTTCTTCCAGGCTGATAAAGCCATGTTTCGCCATGAGCGACAATACGGTATCGCGC CGTTTTTTCGCCGCCTCTGGATGGTCATACGGATTATACCGGTTTGGACTTTGCGGCATGCCGGCGAGCA AGGCCGCTTCCGGAAGAGTGAGCTTTTTCAAGTCTGTCTTGCCAAAATAATATTCGGCGGCGCGAGCGAC GCCATAAATGCCGTCTGAATAATAAATTTTGTTCAAATACATTTCCAAAATTTCATGCTTCGAATATTTT TGTTCAAGCTGCAGCGCGAGCCATGCCTCTTGCGCTTTTCGCTTCAACGTTTTTTCTGGTGACAAAAACG TCATTTTGACGACTTGCTGGGTGATCGTGCTGCCGCCTTCTGCACCAAATCCTTCCTTGATGTTGGCGAG GACGGCGCCAGCCAAGCGGACGACATCGATGCCGTGATGTTCATAAAAGCGGGCGTCTTCCGTTGCCAGC ACGGCATCTTCAAGCACTTTGGGCACGTCTTTATACGAAATGTACGTCCGCTTGTAGCCGCCAAGTTCGG CGACTTTCTTCCCATTCATATCGCACACGATGGAAGAGAGCGGGTCTTTAATTTTCGCCTCATCAAGCGG GGGAGCGTCCTTGACGAAGTAGGCGACCGCCGCCACGCCGCCGACAGCGGCGATCACGATGATGAACAAT ATCGCAATGGCGACTGTTTTCAGCCACGCGATCCCTTTTTTCGTTTTCGCTTTCTTCTGTTTCGTTTGTT TCGCTGCCTGCTTTCGTTCTACGCGAGAACGATATTCACCAGACAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 512529-515234	Sequence	GYMC61_0511 - penicillin-binding protein (Protein is coded in complementary strand; from 515234 to 512529)