Sequence retrieval

Genome: Escherichia coli BL21-Gold(DE3)pLysS AG
Start position: 3046504
End position: 3046563
Length: 60

DNA sequence
>NC_012947, from 3046504 to 3046563 (60 bp); Escherichia coli BL21-Gold(DE3)pLysS AG TCTGCGGCCGCTTTTGCTGCAGCGGCTTTTTCAGCTGCAGCTTTTGCCGCTGCCGCTTTT
Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF. 3046117-3047382	Sequence	ECBD_2921 tolA cell envelope integrity inner membrane protein TolA (Protein is coded in complementary strand; from 3047382 to 3046117)