Sequence retrieval

Genome: Brachyspira hyodysenteriae WA1
Start position: 683050
End position: 683226
Length: 177

DNA sequence
>NC_012225, from 683050 to 683226 (177 bp); Brachyspira hyodysenteriae WA1 TTATATTATTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTT GCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGCTTGC TTGCTTGCTTGCTTGCTTGCTTGCTTGCTACAAGCAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 683050-683226	Sequence	BHWA1_00574 - hypothetical protein (Protein is coded in complementary strand; from 683226 to 683050)