DNA sequence >NC_011993, from 3819426 to 3819656 (231 bp); Escherichia coli LF82 AACACTGCTGATATCGCCACTAACACTACGGATATTGCCACCAACACTGCCAATATCGCCGATAACACTG CTGATATCGCCACTAACACTACGGATATTGCCACCAACACTGCCAATATCGCCGATAACACTGCTGATAT CGCCACTAACACTACGGATATTGCCACCAACACTGCCAATATCGCCGATAACACTGCGGATATCGCCACT AACACTACGGATATTGCGACT Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI