Sequence retrieval

Genome: Streptococcus pyogenes NZ131
Start position: 1410242
End position: 1411753
Length: 1512

DNA sequence
>NC_011375, from 1410242 to 1411753 (1512 bp); Streptococcus pyogenes NZ131 TTAGTTTTTACTCTTGCCAATAACATATGTTAGCTGTTCAGCCTGCTGAGCTGTAAAGGTACGATAAGAA ATCTTACCTAAGCCGATACAGTTAGACTCTGAAATAAGAATAGACCCATCTTTTCTAACATCTTCTACAA TTGATACGTGGCCATAAGTGCCATCAGCGCCTGCTTGCCCGGGTGCAAATGAAATAGCATAACCTACTTT TGGAGTCTTTGACAGGGCATACCCTGCTTTATACTTCCAATCTCCACCATTTCCCATAAAAGGATCAAAT TGGTAACCCAATTCTTTGGCGCGATTATACACATACCAAGTGCATTGTCCCCAAGAATAACTCGAAGCTT GATAACCCAAGACATCATAACTCTTAGAGAGCTTATAACCTGCTGGCACAGATGCAAAACTAGCTGATGA CTGCACTTTCAATTCGGCTGGAATGGCTGGTGTGCCACCATGGTCATCAATCCACTTATCCAGGTCTTCC ATAATTTGTGCGCGACGTTTACCACTACCAGTAGTATCAGTAAAATAAACACCCCCGTCACTTGCAAAGT TTAATTGACCTCGTGAAAACTTAGCCGCTTTTAAATCTTGAAGTGCAAAATTGCTATTTTTGTTTTTAAT AATGGTATCTTGTGTCATTTTGACAATAGCACTATCATCATTAAACTTACTTGCTTGAGTCGGATCTAGA TCAAAAGCTGCATAGCCAAACATATTAGCTCCAAGCAAAGTCGCAATCCCTTGAGTTCCTAAAGAACTCT CAGACATCGCAATAGCTACGATAGCTCGAACATCCAGCCCACTTTTCTTTTCCCATTGCAATAGCTTCTC ACCATTAATACGTGTGCTGTCATAGGCAATGCCTGTTGATTTTAAGTAACTATCAATCTGGTCAGCTTTA ATACCATAACGGCGAGATAAAAGATGGTGGGTATAGGCATTTTTACCACTCCAATGCTCTACATATGCCG CGTATGTGTTGACAGAAGGGATATCTATCTCGCTCAAATCTAAATCTGATACGTAAGGTGACAACGACTG AATAGGAGTTCTTACCTCTGGCAGCTGTCTCGACAATGAAGAAGCAGGTGCCGTATCAACTTTAGATGGT GATGGTTTGGGCTGATCAGTCGGTTTTGGCTGGTCAGTTGGTTTCGAATGATCAGTTGATTTATCATCTA CCTGGTTTTCTGCTTCTGCCTTGTTGTCCGAAGAACCTACAGCAGACTGACTAGAATCGCTTCTTTTTTT ATCATCCGCGTTAACAAGACTATTAGAGTTTGTTTCTGCAGAAGCATCAAGGGGACCTTGGTCAGTTTTA TCTGATGTTGTAGGTGCTGCATTTTTATTATTTTCCTCTTTTGAAGAAGAATCACTGGTTTCATTTGTAG TTGCTTTATGCGTCTCAAGCATTACATCTTGAGCCAACACTGTCATGCTTTCTGCTGTCGGGGCTAAGAG ACTTAGTAGCATGGCAACTCTTAATAGTTTGTTTTTATTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 1410242-1411753	Sequence	Spy49_1407c isp2 Immunogenic secreted protein (Protein is coded in complementary strand; from 1411753 to 1410242)