Sequence retrieval

Genome: Gluconacetobacter diazotrophicus PAl 5
Start position: 778215
End position: 781316
Length: 3102

DNA sequence
>NC_011365, from 778215 to 781316 (3102 bp); Gluconacetobacter diazotrophicus PAl 5 ATGATTAACCAGTTCATCGCGATCTGCCTGCGGCAGCGGCGCATCGTCTTTGCCGCCATGGCCGTGCTGG TCGCTCTGGGTGCCCTGGCGTGGCACCAGATCCCGGTCGAGGCCTATCCGGACCTGGGCGCGGTCAACGT CCAGATCACGACCCAGGTTCCCGGCCTGGCGGCGGAGGAGATCGAGCAGCAGATCACCATTCCGCTGGAA CGCCTGGTGGCGAATACGCCGGGGCTGGTGGACAGCCGATCCAGTTCCACCTTCGGCCTGTCGCTGATCA CCCTGATCTTCCGTGACGGTACCGACGTCTATTTCGTGCGCCAGCAGGTCGCGGAACAGATGGCGCAGGC CACGCTGCCGATGGGCGCCACGCCCTCGCTGGGTCCGGTCACCGGACCGTCGGGCGAGATCTACCGCTAT ACGCTGCAATCCGACAGCAAGAACCTGATGCAATTGTCGGACATCCAGAAATGGATCGTCATCCCGGCCC TGATGCAGGTCCAGGGCGTGGCCGCCGTCAATAATTTCGGCGGCTTCACCATCGAGTACCAGATCGTCCT GAATCCCTCCACGCTGTTCCGCTACGGGGTGGGGGTCAACGACGTCATCACCGCATTGCAGGCCAACAAC GCCAATGCCGGCGGCGGCCGCATCACCCGGGGCGAGCAGTCCTATATCGTGCGTGGCGTCGGCATGGTCC ACACCATGGCCGACATGGGCGCCATCGCGGTGACGCAGCGCAACGGCGTGCCGGTCCTGATCCGCGATCT GGGCCAGTTGCAGCTCGGCCACCAGGTGCGCGAGGGCATCCTGGGCATGAACGGCAATTCGGACACGATC GAAGGCATCGTGACGATGCTGAGCAAGCAGAATCCGTCGCTGGTGCTGCACGACCTGCACGGGGTCATCG ACCAGTTGCAGGCCCGGCTGGCCCCGATGGGCGTGCGGATCGTCCCCTATATCGACCGCGACCGCCTGGT GCAGACCACGACCGACAAGGTCACCGACACGGTGGTCAAGGGCGTGGGGCTGGTATTCCTGATCCTGGTC CTGTTCCTGGGCAGCCCGCGCAGCGCCATCGTCGCCGCCGTCACCATTCCGCTGGCGCTGGCGACCGTGT TCGTGGTGATGCACCTGCTGGGAATGCCGGCCAACCTGTTCTCGCTCGGCGCCATCGATTTCGGCGTGAT CGTGGACGGCGCCATCGTCGTGACCGAGGCCATCCTGCGCATCCGCGAGGAACACCCGACCCACACGATG GACGAGGAGGATATCCTTGCGGTTACCAAACATATCGGCAAGGCGATCTTCTTCGCGACGCTGATCATCA TCGTCGCCTATGGCCCGCTGTTCGCGTTCGAGGGCGCCGAGGGAAAGCTGTTCGAGCCGATGGCCTTCAC CGTCAGCTTCGCCCTGCTGGGGGCGCTGTTGTGCGCCATCACGCTGACGCCGGCCCTGGCCTACCTGGCC ATGCGCAAGCCGCACCGCATGTTCCACAACGTCCCGCTGGAAAAGCTGCACCATGCCTATCGCCGCTGGC TGGGCCATATGGTGGACCGGCCCTGGGCGGCCTATCTGGCCGGGGCGATCGCCTTCGCCCTGGTGCTGTG GCTGGGCGGGCGCACCGGCCGCGAATTCCTGCCCGACCTGGACGAGGGTGCGCTGTGGCTGCAGATCCAG CTTCCCTCCGGCCTGTCCCTGGACAAGGCGGGTTCCATGGCGGACGAAATCCGGGCCGCGATCCATGCCT TTCCCGAAACCAGCTATGTGGCGACCCAGCTTGGCCGCAACGATTCCGGCACCGACCCCTGGAGCCCCTC GCACATCGAGGCACCGGTCGGCCTGACGCCCTATGCGACCTGGCCGCACGGCGAGAACCGGGTGCAGTTC GTCAAGCGCCTGCATGACCGGCTGGCGCAGATCCCGGGTATCAGCTTCGGCATCAGCCAGCCGATCGCCG ATAACATGAACGACCTGGTCGGCGGGGCGCACAGCCCGCTGGTGCTGCGCGTCTATGGCGACGATTTCAA GGAACTGCGTCGGATCGGCAACCAGGTCGTCGATATCCTGAAACAGGTTCCCGGGACCGCCGACGCCTCG ATCTTCCAGGAACCCGAAATCCCGCAGATGGACATCACCGTCGACCGGGCCGCGGCCGCGCGCTACGGAA TCACCGGGACCGACGTGATGAACGTCGTGCAGAACATGGTCGGCGACGCCCCGGTCGGGCAGGTCTATGT GGCCGACCGGATCTATTCCATGACGGCGCACATGGAACGGCAGTTGAACAACAACCTGCCCGCCATCAAC CAGATTCCGCTGACGGGGCTGAACGGCGCACGCATCCCGCTGGGGCTGGTCGCCCATGTCTCGCTGCAGA CCGGCGAGGCCAATATCGCGCACGAGATGAACCATCGCCAGATCACCATCCGCGTCGATAACGGCCAGCG CCCGCTGTCGCAGTACCTGGCCGACGCGCAGGCCCGCATCAACAGCCAGGTCCATTTCGACGCCGCGCGT TACCATCTGGAATGGGCTGGCACCTTCCAGCAGGAACAGCGCGCGCAGGCCCGCCTGACGGTGGCGCTGG GGATCATGTTCGCGGTGATGCTGGTGCTGCTGTTCGGCCAGTTCGGGCGTATCCGGCAGGCAGTGCTTGT GCTGGCGGTGGTGCCCATGGCCACCCTGGGCGGGCTGGTGGCACTGAACCTGCGCGGCGAGACGCTGAAC ATCGCCACCGCGGTCGGATTCATCGCGCTGTTCGGCGTCGCCATCCAGAACGGCATCATCATGGTGTCGG CCATCAACCGCCTGCGCGACGAGGGCATGCCCCTGCGCGACGCGGTGCTGGAGGGGGCGAGCGAACGGTT CCGCCCCGTGCTGATGACCGCGACCGTCGCCAGCATGGGCATGCTGCCCGCGGCGCTGGCCACCGGCATC GGCACCGACGTGCAGCGCGGGCTGGCCACCGTGGTGGTGGGGGGGCTGGGCATCGCCACCCTGCTGACCC TGTTCATCCTGCCCACCTATTATTGCGAGTTGGAAGAATGGTGTGCCCGCCGCGACCCGCGCCGCGAAGC CCGCAAGGGAGGCGTGACATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 777080-778222	Sequence	Gdia_0686 - RND family efflux transporter MFP subunit
ORF.. 778215-781316	Sequence	Gdia_0687 - CzcA family heavy metal efflux pump
ORF... 781313-782830	Sequence	Gdia_0688 - NodT family RND efflux system, outer membrane lipoprotein