DNA sequence >NC_011365, from 2255475 to 2255546 (72 bp); Gluconacetobacter diazotrophicus PAl 5 GGGCTGGCGCGGCGGATGGGGGCCTGGCTGGCATGGCGGATGGGGACCCGGCTGGCGTGGCGGCTGGGGG CC Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI