DNA sequence >NC_010682, from 315922 to 316071 (150 bp); Ralstonia pickettii 12J AAGGATGCGATGTCGAAGGACGCGATGTCGAAGGACGCGATGTCGAAGGACGCGATGTCGAAGGACGCGA TGTCGAAGGACGCGATGTCGAAGGACGCGATGTCGAAGGACGCGATGTCGAAGGATGCGATGTCGAAAGA TGCGATGTCG Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI