Sequence retrieval

Genome: Yersinia pseudotuberculosis PB1/+
Start position: 531203
End position: 535141
Length: 3939

DNA sequence
>NC_010634, from 531203 to 535141 (3939 bp); Yersinia pseudotuberculosis PB1/+ ATGAAGTGGGTAAAGAGACTCAGTATTGCATTCTTGCTGATTATCCTGCTATTGGTCGGTGCTCTGGGGG GGTTACTCGGTACGACCAGTGGGTTACATTTTTTAATCAACAGCGCCGCCCGCTGGGTACCTGGGCTTGA TATTGCCAGTGTTAGTGGTGGCTGGCGTGGTCTCACCTTAACGGGCATTCAGTATCAAATGCCCGGCGTC ACGGTCAAAGCTGGGCAATTTTATCTGTCATTGCAGCTCTCTTGTCTGAAGAATAGTGAGCTATGTGTTA ACGCACTGACAGCACAAGATATTGATGTGGTGGTGAATACTCAAGCGATGCCACCTACGTCATCCACGCC AGCCAGTACTGACCCCATGGGGGAACTGAGTACCCCATATCCCATTTTCTTACGTTTGCTGTCACTGAAT AACGTCAAAGTGACCATTGATGATACGGCTATTTCACTGGATGAATTCCGTACTGGTGCGCATTGGAAGC AACGTTCATTGGCTTTGATGCCGACAAAAATCAACGGGTTATTGATCGCATTACCCAAAACGGTGTCAGC CGGGGTTCCCGATGCGGTGAAGCCTGCGGTTGAAACTGCTATGGCCGCCAAAGAGGCGGTTGCGCAATTA TCAGCGGCTCCTGGACGATCAGCCACTGCATCAGGGCGATCAGCCACAGCATCAGGGCAATCAACCACTG CGTCAGGACGATCAGCTACTGCGTCAGGGCAATCAGCCACTACCGGGGGTCAACTGGCTACGGTCCCAGC AGAACCGGCGACCCCACTGGGTGAAAGTTTAAAAGCGCTATTTGCCAAACCATTGCTACCGGCATTGCCG GACTTCCGTCTACCGCTGGATTTGCAGATTGAAGAGATCTCAGGGCAACAGCTTAGGCTGACCGGGGATA ACGAGGTCCTTATCAGCAGCTTGTTACTGCAGGCCAGTACACAAGATCAACGGATAACACTGGATACGCT GGAGATCAAATCGCCGCAGGGCGGGTTGTCGGCGCAGGGGCAAGCGACATTGGCGGATAAGTGGCCGCTG GATCTGGTGGTCAATAGTGCGTTGAACATTGACTCGCTGAAAGGCGAAAAAGTGAAATTGACCGTCGGTG GGGCGCTGCGTGAGGAACTCAAGGTCACGCTCAATCTGTCAGGGCCGGTCAGTGCGCAACTGGAGACAGA AACGTCGCTGGCAAAGGCAGGTTTGCCATTGTCGCTGACTCTGCAGAGCAAACAACTGCGCTGGCCGTTG ACTGGCGAGCCCCAATACCAAATGAATAACCTCAGGATGCGCCTCAATGGGCAAGCGACGGATTATGCGC TTTCTATCCGTTCTGATATCAAAGGTACTGATCTACCGCCAGCGGTATTCACGCTGGAGGGTAAAGGCAA TGTTGAGCAATTTAATCTGACGCGTTTACGTCTGGCGGCGTTGCAAGGTCACACTGACCTGACTGGGGTA GTGGATTGGCGGCAAGCGATCAGTTGGAATTCGGTACTCACCTTATCGGGCATCAATACGGCCAAACAGT GGCCTGAATGGCCAGCAAAATTGGAGGGTAAGATTGTTACCCGCGGCAGTATTCATGGCGGTAGCTGGCA ATTGCAGGTTCCGGAGCTGGTACTTGATGGCAATGTGAAACAGAATCGCGTGACCGCCCGCGGGTCTCTG ACCGGTAATGCGGCGGGCCAATGGCATATTCCTGGTATCAATCTGGCGTTGGGCCGTAATAAGCTTGATA TGAAAGGGGAGTTGAACGACAACTGGCTGTTGGATGCTAATGTCGATGCGCCACAACTTGATGGCGCGTT GCCCGGTCTGGGTGGTGTAGTGAAAGGGTCGCTGAAATTACGCGGTAACCTGAAAACGCCGCAACTGCTG GCTGATCTCACGGCTAACCGCCTGCAATGGCAAGAGTTGCACATCAATCGTATTAAAGTGGATGGCGATG TCCGTTCGACAGATCAAATTCAAGGCCAGTTGGCGGTCAGAGTTGAACAGCTTAAGCAAGCTGATTTTGT GGTCAGTTTGTTGACGTTGGATGCCCGTGGCAGTGAAAAACAGCATCAGTTGCGTCTGAATATGCAAGGG GATCCTGTCTCCGGCCAATTGTCGCTGGAGGGCAGCTTCGACAAACAACAACAGCGTTGGCGCGGTACTC TCAATAACACGCGCTTTGATACCCCAGTGGGGGAATGGCGTTTAAGCCGTGCAATGACACTGGATTATCA GAACAGCGCGCAACGGATCACCATCGGCCCCCACTGTTGGCTTAATCCCAATGCTGAACTTTGTGCGCCC CGGCCAATTGAAGCTGGCCCCAGTGGGCAGGCAAGTGTGGTACTCAACCGCTTTGATTTAGCGATGATCA AACCGTTCCTCGGCCCGGATACCACCATGAACGGTGTGTTTACTGGCCGGGCAGAGGTCAGTTGGAAAGA GGGCGGTAAGTTACCTGATGTGCGGGTATCACTCAGTGGTAATGGCGTGAAAGTACAACAAATGGTGCAG GGCAGCCCACTGCCGATTGCCTTTGAAACCCTGAACCTGAATGGCGGGCTGGCGAATGGGCAAGTGCGCG CCGACTGGTTGGTCAAGCTCGTCAATAACGGGCAGTTCTCTGGGCAGGTGCAAGTTGCAGACCCAGAAGG GCGGCGCAACTTATCCGGTAATGTAGCCATAAGTAATCTCTCTCTGGCGATGATTAACCCGATTCTGAGT GATGGTGAAAAAGCGGCTGGCATATTGAATGCGAATCTGCGTTTGGCAGGGAATGCGAAAAGTCCGCTCG TCTATGGCCGCCTGGCACTGGATAAGGTGGATATTGATGGCAGTTGGATGCCATTCGATATCACCGAAGG GCGCTTGGCGATGAATTTTGATGGCATGACATCGACGCTGGAGGGGTTGATACGGACTTCCCAAGGGCAG TTGAATCTCTCCGGCGATGCGGACTGGCGTGATATTAGCGCCTGGCATGCCAGAATTGCGGCCAAGGGCA ACAAGTTACGGGTTACGATCCCGCCGACGGCGCGAGTCGATGTTTCACCGGATATTGTTTTCGAAGCCAC GCCGCAGTTATTTACGCTCAATGGTTCGGTCGATATTCCTTGGGCGCGCATCACCGTGCAGGAAGTGCCT GAAACAGCGGTTGGCGTCTCCTCGAATGAGGTTATGCTCAATGACCAATTGCAGCCTATTGCGCCACAGA GCGCCAGTATTCCGATCAGCAGTAATTTGGTGATTCGGATCGGTAACGATGTTCGTTTGAATGCATTTGG TTTGAAAGCCCGCTTGCAGGGCGATTTGAGAATGATGCAGGATCAGCGTGGGTTAGGGCTGAATGGGCAA ATTAATATTCCGTCCGGTAGCTTCCGCGCTTATGGTCAGGACCTCATCGTCAACAAAGGAATATTGCTGT TCTCTGGCCCACCAGATCAGCCGCTACTTAATATTGAAGCGATCCGAAATCCTGACTCTACCGCTAATGG TGTTATTGCCGGAGTACGTGTTACGGGGATGGCAGATTCCCCGCGCATGGAGGTATTCTCTGATCCGGCA ATGTCTCAACAGGAGGCCTTATCTTATCTGTTGCGTGGCCAGGGGCTTGGCAATTCGGGGGCTGACAGCA GTATGATGACCTCAATGTTGATCGGCATGGGGGTGGCACAAAGTGGTAAATTGGTGGGTAAAATCGGTGA GGTATTTGGTGTCAGTAATCTGGCATTAGATACTCAAGGGGTTGGTGATAGCTCACAAGTGGTCGTGAGT GGTTACGTCACCAAAGACCTGCAAATAAAATATGGTGTGGGTATATTTGACTCACTGGCTACGTTAACGT TACGTTATCGGTTGATGCCCAGGTTGTATCTGGAAGCGGTGTCTGGTATTGATCAGGCATTAGATTTGCT TTATCAGTTTGAGTTTTAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 529470-531206	Sequence	YPTS_0482 - surface antigen (D15)
ORF.. 531203-535141	Sequence	YPTS_0483 - hypothetical protein