Sequence retrieval

Genome: Yersinia pseudotuberculosis PB1/+
Start position: 427704
End position: 431522
Length: 3819

DNA sequence
>NC_010634, from 427704 to 431522 (3819 bp); Yersinia pseudotuberculosis PB1/+ TTACCAGCTCACTCGTGCACCTATCGTGCCGCCGACGGCATAGTTGTCACTGACATTCCGTGTAGCCCCC AGCAGATTGACATTGCCGTAAACCGCGTAGCGACCATTCTGCCATTCATACGTACCGCCCGCGCCTAACC CCACCGACTGACGCTCATCCCGGGTGGCGAACTCCACCCCTGAGACCTGTACTTTGCTGCCATTGAGGAA CTCATTGTGCAGATCGAGATGACTGTAAATGTGTGAGCGGCGGGTGGTGCCGTCTTTCGCACTCCAGGTC GTTTCCTTATCCAGTGAGACACCGAGGCGGCCCCGCAGGCTGTCACCCTCTTGCAGCGAGACTTCGCTAT CAAACGGATCGCGGAAGGTATCGAAATCGACCCGCGAGTAGGTCACCTGCATCTGCGGAGTCAGCGATAA CCCGTTACCCAGTGCGTAACCTTTACCGGCTTCTATCGCCGAGGTATACCCACGGCCATTATTGCCACTG GCCACCGCATGCCCTGCGGTACGGGAGCTCAGGTCGCTGTCAAACCACATCGTCTGCAACTGGCCATCCA CATACACGCCGTTATTGCCATACCAGGTCAGGCTGGTGCCAAGACCGTAACCTGCGCTGTTGATGCGGCC ATCACCAAAGAATGAGTGGATATCCGCTTTGGCTTTTCCGTAGGTGAAGTTCACCCCGCCGGTCAGCAGT GAACCGCCCTGGCTCTGATACAACGGCACATCAATGCCGGTCTGCAACTTCCACACATCAATCTCACGCT GTGAACCGCTGGTGGATCGGGCCGGGTCGGTGACCTGGTGGCTCCCTTCAATACGGCCCCAGGCCCATTG ATTATCGTCAAAGTTCAGATCTGCCAGTGCGCCCGGTGCCCCGTAACGGTTACCGACACGCTGTTGCAGC GTCGGCAGGGTATTCAGGGCGGCCAGCACCTGCGGATATTGTTCATACAGCGGCACGCCCACCTGATAGC GTACCCCCTCGGTTAACATCAGTTCTGAAGAGAGATACCAGTTGCGCCCCGCAGTGGCGGCTTCCCCGTC GGCCTGTAAAGTGTAGGCATACGCCCCGGCCACCACCGCCTGATAGCCATCCGTGGTGACATAATCCCCG GCCAGCAGGAAGGTGTCATCCTGCGCCAGACCGTCCACGGTGATCAGATTGATGCCATTGAGGGTTTTAT CGCCCAGACCGCCCGCATTGTTCACCCGGACCGAGGTGGTGCCGGTGGCATCGCCGTTGATCACTAACCG GTCGGTGGCAGAATCGTCATCCCCCAGCACCGTATCGAGTGCCAGCCAGGCCCCTTCACCGGAACCGTTA TTTTCACCAAAATAAGTCCCGGTCACGGTCAGCCGATCGTCGGTAGCGGCACCATTGCCGCCGGTATAGG TGTTTGACAGCAGGATCCCACCGGCGCTGATGAGGTTGCCGCTCAGGACCGAGTCACCGAGTGAGGACAG CGCGCCGCCTTCAGCCAGGGTTAATGCACCGGTTTCGGTAGCCACTAGCGTCGGGTCAGCGGCGATATCG GCGGCACTGAGGCGATTTAATATGGTGGTGGCAGGCGTCAGGCCCAACGTGGCGTTATCCAGCACCAAAA TCCCCCCGGCGATATTGGCCGACAGGAAGGCATTGGCGTCACCGTCAGCCATATTGGCGCCGGTTAACGT CCAGATACTGTTATTGGTTTTGGTCAGGGTACCGAAGCCGGTGATCCGGTTCGGGTCGCCATCCAGAATG GCCTCTTCACGGTTATCAATACGCGTGAGATCGAAAGCAGCGTCCCCTGTTCCCCCCAGCACCAGATGGC TGTTTGCCAGATCCAACGCGGCATCATTGATCTCCGCATAACTGCCATTGAAGACCAGTGCCTGCGTAAC GCCTTCAAGGGCATAGCCAGATTGCAGCTCTAACGTGGCCGATGTTTGAATGTTATCAAGCCGATGAATG CCGATTGCGGTGCCATTGCCGCCGTGCACCAAGCCGCCCACGGTGACATAGGTGTCTGCGGTATTGGTTT CAATATTAATTCCGTAGCCCTGCTGCGAAACCAGCGCGCCCCGGGTGATCAAATCAATGGTCGAGGTGCC GTTGTCTGCGCCTGTGCCATCATCCACGCCTGTGGCGTAGTTATCATTATAGTTATAGTTATAGTTATAG TTATAGTTATAGTTATAGAGGCGTATGGCATCGTATTCACTGGCAATATTATTGACGTCAACGTTGACCA AAGTGCGGCCATCAGTGGTCAGTGCATTAAGGGTAATCCCCTCTCCGTGGCTGACGTTAATCTGGCCCGT CACGGTGATATCGGTGGTTGAAGTACCTTGGTTAGCTTCGTTAATGAGAGATATTCCACTGCTCATTGCA GTGATGTCATTGAAGTGGATGAGCGTGGTGGCATCCCCCAGAGAGGAATGGGTCTGGAGCATTGCCCCTT GTCCCCATTCCGCTGTGAGATGCCCGGTCGCGGTAATGGCAGAAAAAGCTGAACCCAAAAAATTGTAATT GTTGATATCAAGCGATTGACTGCTGGAAGTAATATTATTCACATTGATGAGCATAGTAGAACTGCCTTCA CCAGCCGCGCCCATCACTTTAATTCCGGTACCGTTTTCTGCAGTAATCGTTCCTGAAATATCAAGGTCAT GCAGTATATTTCCGGATCGGGTATAGTCGCTAATATTGAGTGCATCATACAAAGAATAAACGTTATTCAA CTTGATAGCGGTCTCGATGTTTCCCTCATCCGAGTAGGCATTGGCTTGTAGAGCAGATCCTGAAGTAGCC GTAAGATCACCCGTCAATATAATATCGGTTACAGCGCCATTTTGGCTGCTATTATCGATATAAAGGCCAG TCGATCCAGTGACATTATTTGCGCGAAATCTAAGGCTCGCAAGACCGCCATTGGTATAATTGTAAATCCT GGCTGCTTGACTATTAGCTGAACTGATATCGCCATCAATATTGATATCAGTTATCACTCCACCTACATAA TTGGTATTTTGAATATAAAAACTGTCATATTCTGTATTAATACTCTTTGAATTGAAGTTAATTATGCTGG TGCCACCATTGGCAGCATTTCGGATCGAGACGCCTGAATTTTCGACGTTAATATCACCAGTAACATCAAC GTGGGTGATGGTCGCTCCATTTTGTGAATTATTATCGCTTTGGATGCCAGTAACACCTGAAATAGCGTGG GTGTTCAGAATGATAGTTGAATCATTGCCTGCCGATGAAAAGATTGAAAGGGCTGGAGCGCCGCTCAGGC TGCTGGATAAGATGCCAGAGAGATCTACCCGTATGGTCGAATCGTCACTGCTCTGGTTGTCAATGTAAAC GCCACTGCTAATGGAGCCGTTGCTCTGAACATTAATGGATGCCGATTGCTCGGTTATTAATGTGTCATTC TTAATATACAAAGAATATCGACTGTCGGTATCCAGTGCAGAACTGTTGGTATCGAGGTAACTGATAGTAC CCGAACCGGTTAACGAGAGCGCAGAATCCATAATTTCATGTTCTGTTATGCCAAAACCGGGCCGGGTCTC AACGGCGATATCCGTGCCGAACAGGGATATCGCGGTGTTATTTTCACCTTCGCAAACATAGGTTCCCACC CCCGAGCTATTGCACGCCGCCCATGCGGTTTGTGGTAAGACGAGGGTGGAGAACGCAACAATAATTGCCA ATGTAATCGGATTCATCACGGCTGCAGGAACACGGCTGAAGAGGTGTTGACGCCCTGCATCCGGTTGAGC GGGTGAGGTAGAAATAGGCTCTCTGAATTGTATATGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 427704-431522	Sequence	YPTS_0388 - outer membrane autotransporter (Protein is coded in complementary strand; from 431522 to 427704)