Sequence retrieval

Genome: Yersinia pseudotuberculosis PB1/+
Start position: 270286
End position: 270804
Length: 519

DNA sequence
>NC_010634, from 270286 to 270804 (519 bp); Yersinia pseudotuberculosis PB1/+ TTAACTATTAACTTCGAATACCAATGTCAGTTGCCCGTGATATTTCCCTGCAGTATTGTCCCCTGCGGGG GCTTTTGCGGATATATGTAAGACATAATCGGTGGTGGTGTGGCGGGTTGCCTGTGGAGGTACGCTAAAGG ACTCCGGTACCGCTGACAGTAAACGTAAATTGGTGCGGTTATTTCCCCAACGCACTTCCGCCGGTGAGAA TGTCTGTTCTATTGCGCTGAAAGCATCTGATTGGCGGGTGAGTATTAGCGGGTTTTTAATTGAGATTTGA TACCCCTCTTGCCGCCGGAGTTTGACGGCTACCGGGACTTCTACCTCATAAACGGCATGGTTCTCCGGGC TATTGTGCAGCTTGATATTGTCGTACCAGCCACCATGCGGTTTTTCGATTTCGATTCTGGGTTGGGCTGT TACCTCTAATTTGACGTCGATATCCCCCAATGGCGTTGCGGCCCAGCAGGTGTGCCATGAGAGCAATAGC AATGTGATGGCCATTTTTATGGTATTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 270286-270804	Sequence	YPTS_0248 - hypothetical protein (Protein is coded in complementary strand; from 270804 to 270286)