Sequence retrieval

Genome: Burkholderia ambifaria MC40-6 chromosome 1
Start position: 471529
End position: 475470
Length: 3942

DNA sequence
>NC_010551, from 471529 to 475470 (3942 bp); Burkholderia ambifaria MC40-6 chromosome 1 TCATTGCGCCCCCTGTGCATTCGTTGCGGTGGCGGCCGTGACGGCCGGTGCCGCGGCCGGCGCGCCGTTG CCGGCCTGCGTCGCGTTCTGTGCCGCGCCCGCGCTCGCGTCGGCGACCGACGACGGGAGCTGCAGGCCGC GCAGGCTCTGCTGCTGCGGCTGGTCGCCGCCGCCGCCCGTCGGCTGCGACGTGCTGATGATACGCATCGT CACCGACACGTTCACGCTGCCCTGGACCCACGTGAGGTCGAAGGTGCCGTCCGGGCGGCGCTTGCGCTGC GCGGAGTTGATCAGCTTCTCGAGACCGTAGCGGCCCGGCTCGTTGACGAGCTGCACCGTGCGGCCGTCGA AGGTCGTCGCGGACAGCGTCGCGCCCGGCGAACCCTGCGGGTTCGGCCACACGAAATTGGTCCACTGCGG CGGCGTGTTGCGGTAGCGCAGTTGCTGGCCGTCGATCGCGATCGTGTATTCCGTCGTGCCCGTGCTCGGC TGCGGCAGGATCTGGAACACGGTCTGCGGTTCGGCCGCCGCGGCCGCGCCGCCTGCGGCGCCGCCGGCGA GCGGGGCGACCCAGCGCGCGAAGCCGTTCGTGAAGTCAGGCGTGAGGCCGATGCCCATGTCGCCCCACGT GCGGGCCGCGAGCGTGTCGCCGCGGCGCACCGCGAGCGGCCCCAGCGTCGTGCCGACGAACTTGGCGATC GCACCGTCCGGGCCGAACACCTGCGCGATTTCACCTGCGCCGGCCTCGACCTTCGCATTCGCGGCGAACG GGTACTTCGTCGCGAGCGAGCTCTGGAACGGCTGGTAGACCTGCGCGTTCCACACCTTGTTGACTTCGGC GCTGGCCGGCTGGATCACCACCGAGAACGCCTGCATCAGCGGGCGCACCAGCAGCGGACGCAGCGACTTG CGTTGCGAATCGGTGAGGCCGGTCAGCATCTGCTCGTCGACGAACTTCAGCGAATCGGCGAGTTCCGAGC CGTTGCCGTCGAGCGTCTGCTGCATCAGCTGGCGCGCGCCGGGGCCGGGGTCGCCCTGGTTCTTGATCAC GTTGAAGCGCGTGCGCACCTTCGACAGCGAATCCATGTAGCCCTTGAGCATCGACGTGCCGTCGTGCGTC GCGACGATCCGCGCGAGCCCGATGAACTCCTGGCCGATCGGACCCATCGGCACCTCGGCCGGATTGCCGT TGATGTCGATGTTCGCGGCCGCCATCTGGCCGGCCTGCGAGCGCGTGAACAGTTGCTTGACCCAGTTCAC GACACCCGTCTGCGCCTTCTTGATCGTCACGTTCGCGAGCGACGGGTTGTCCCACGACGTCTGGTCGTAC GCGGTCTCGAGGATCTTGCGGATCGGCGAATCCTGCGGGTCGCCGAGGCGGTTCATCCCGTCGACGGCCT GGCCGAAGCTGCTGAAACCCTGCACCGCGATGCCCTGCATGAACTTCTGCCAGTGCTGCGCGTACTCGGT CTTGTACATCCCGACGAGGGTCTTCTGGATCTGCTCGGGGCTGCCTTCCAGCGTCAGGTCGTCCTGCGTC GACGTGTTCAGCACCCAGTCCTTCGCCTGCAGCTCCTTGGTCGCCGCGTCGCGGATCGCCGGCTGCACGT AGTCGAACCATGCTTCACGCGTGAACGTGCCCGGAATCGCGTAGCTGCCGGCGACCAGTCCCTGGTTGCC GTCGCCGACGATGCGCGCGATGGTCATCGGCGCGAAACGGGTCGACGCGCGCGCCTTGATTTCCTCGTAG ACGCGCTGGCGGGCCGGCATGCCGCGCACGACGCGGCGCAGGTTCTCGCGGGTCTGGTCGACGAGCGCGA GGTTCGCGTCGATCATCGGCCAGTCATCGTCGGTCACGCGCGACAGATAGAACGAGATCATGCGCTCGGC GCTCTTGATCATCTCGTCGCGCGGCATGTTGCCGCGATTCGTCTCGAGCCAGCCGCGCCAGAAGCGGGCG AGTTGGTCGGTCAGGTGCGCCTGTTCGACATGACGCTTGTCGGACAGCATCAGGTAGGTCTTCAGCGCGT TGTACGCGTCCTGCACGTTGGTCGGCGACGCGTCGCTGTACAGCCCGCCCTGCGGCGCGGCCGGCTGTTG CGGCGCGGCGGCCGTCGCGGCGGATGCGCCTTGCGCGGCGCCGGCCGGCGGTGCGGCGCCCGGCGGGTTC GTCGAGACCGGCAGCGCGCCGCCCTGCACGGCACCCGATTCGGGCGGGCGCGTCATCGGCACGAGCTGTT CGGGGTGCGCGTTCACGTCCTTCATGAACGACGCGAGGTTCTGCGAGACCGGCGCGAGCAGGATCTGGCG CACGCCGTTGTAGTACTCGGTCAGCAGATGCTGCTCGAGACGGTCGCCCTGGTACAGGCCGAGCGACACC GATACCGGCTTGTCGCGGCGGAACTGCTCGAGCTGTTCGATACGGTCTTCCAGGATGTCCATTGCCTGCA GGCGCGACTGCAGGTCGTTGCGGCCCTGCTGGAGGCGCGTGACGTTGTCGAGGTCGGCCTGCACGTTCGC GACGAGCTGCTGGTTGCCGATCGTCGACCAGGTCCAGCCGCCGAGGGCGAGCGCGAGGGCCGCGACGAAG CCGAAGAAGGTCGCGTAGCGCAACCGGGTCTTGGTCGGGCTCGCGAACTGGCGCACAGTCTGGCGATCCG CGAAGATCACCTTGGAGAACAGGTCGCGCAGGAAGAAGCCGTTCTTCGAGAACGCGCTGTGCGGCTTCGG CAGCGCGCTCGCGTCGAGGCCGAAGCGGTGCGCGATGCGCTGCGCGGCGGCGCTGTTGGTTTCGCCTTCC TGCAGCGCGCTGGTGAAGTAGAAGCCGCGGAAGATCGGCTTGTACTGGAACGGGTTGTTCTCGAACAGCG TCGCGAGGAACGCGCGCAGCGACGGCTTGATCGTCGAGAATTCGAGCGGGAAGCTCAACTGGCCGGGCGA CAGCTGGTTGCCGCGCGACAGCGACAGCTGCGCGACGCTGATTTCCTTCAGCCCTTCGTACAGTTCCTCG AAGCGCTCGTCGAACAGCGCGACCACGTCACGCTTCTCGTCCGGTTCGTAGGGCAGGGTGGCGCCCCACA CGCGGTCGTACTCGTGCTTGTCGCTGCTGCTGAAGAATTCGGTGAAGCCGGTGATCAGGTCGGCCTTCGT GAACATCACGTAGACCGGCGCGAACACCTCGAGCTTTTCCGTGAGCTCCTGAACGCGCTGACGGAGGTTT TTCGCGAGGTTGATCGCAAATTCGGGGCGGTTGCCGGTGAGTTCGGCGATGCTCGCGGTGACGATGATGC CGTTGATCGGCGCCTTCGGACGGTAGCGCTTGAGCAGGCCGAGGAAGCCGAGCCATTCGCTGCGGTCTTC CTCGTGCACCGAGTAGCGGCCGGCCGTGTCGAGCAGGATCCCCTCGGTCGTGAAGAACCAGTCGCAGTTG CGCGTGCCGCCGATGCCGTGGATCACGGCGCTGTTCTTGTCCGCGAACGGAAACTGCAGGCCGGAGTTCA GCACGGCGCTGCTCTTGCCCGCGGCCGGGTTGCCGATCACGATGTACCACGGCAGTTCGTACAGCGCGGA GCCGCCCGACACCTGGCCGATCTTCGACGTCTTGATCGTCTTCACCGCGTCGGACAGGCGCGTGCGCAGC ACGTCGAGGTCGGCGGTCTTCGAGTCGGGCGCGAGCGCGGCCGCGGCCGGCGCGGCGATCTTGCCGGTTT CCGCCTGCTCCTCCAGCACCTGGCCGAGTTGCTGGTTCGCGCGCTTCACGCGCCAGCGGCGCCACAACGC GACGCCGAGCCACAGCACGAGGATCGCCGCGAAGGCGAGCGCCGCCCACAGGAGCGGCAGCTGCACCATG TCGGCGACGATGAAGAGAATCGCCGCTAGCGCGACGATCCCGACAATCGAGAGCGTACGCGGATGAGTCA GCACGTTGAGGATGCGTTGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 470543-471532	Sequence	BamMC406_0419 - hypothetical protein (Protein is coded in complementary strand; from 471532 to 470543)
ORF.. 471529-475470	Sequence	BamMC406_0420 - type VI secretion protein IcmF (Protein is coded in complementary strand; from 475470 to 471529)