Sequence retrieval

Genome: Polynucleobacter necessarius STIR1
Start position: 350902
End position: 353499
Length: 2598

DNA sequence
>NC_010531, from 350902 to 353499 (2598 bp); Polynucleobacter necessarius STIR1 TTAGACCGTTTTTTCTTGGCCGCCGGTTTCAACTAAAACCATTGGACCGTCAGGAAGTTGGGCGGCAGGT TTAGGTGCTCTACCTAAGTTATGTGCTGCAGGCTCTGCCTGAATTTGGCTTTGCGCTTCTGCGTGCTTTG ATGAATCAGTAGCAACCCAGATCATGCCTGTGGATTGCACAACGCTATGCAGTGGAGTTTCTTCAAGCGC CCGGAAGGCAACCTTTGGAAGTTCTGGTGCAGGCTTGCTGATTACTTGAGCTGCGGCAGCTACGGCAACC GGTGCTGCAGTTTGAGCAACTGGAGCAGAAGCGGGAGCGGCATTTTGCACAGGACGATTCGATTGACGTG GAGCACGTGGTGCGCGCTCTTGTCTCTCTGCAGCAGGTTTTGTATTTCCAAAACTATTAGCAAGGTTCTG AATCGACATGCTCGCAGATGCGCCAGCCATGCCTACAGGAGGGCCTGCAAAGGGGCTTGCTGATACGGCG GCAGCTGATGCAGTAGCTGTATCGTTTGTGCCTTCTGTGCGCTCGCCACATTGACCGCGACCACGGCCAC GACGCTCTTCACCATCAGCGCCCGGAGTAGCTTCGTTGCCAGGAGTTGCTTCGGTTGCAGGTGTTACTGC AGCAGCGTTTGCCTGATTGCGGTTGCCTTCTTGGCGCTCTAGCTTAGGACCATTTTGATTGCGATTGCCG TTACGGTTGTTGCGACCGCGATTATTGTTACCACCTTCTGCTGGAGCACCCTCGGCTGCAGCTGCTGCTG GACGCTCAGTGTGTTCACCGTGACGATTACGACCACGATTGCGATCACTATTACGGCCTTGATTGCGGCC ACGATTATTACTTGGTGCTGGCTTTTCTTCAACCGCTGGAGATGAGCCAAAGAGGCTCTTAATAAATTCA AAGAAACCACCAGTAGTGCTTACTGCTTTTGCTTTTTCAGTGCGGGCAGGGCGTGGTTGGCTAATTGGTG CTGGTTGTGTTGGGGTGATGCCTTTTACAGCAGCTTCTGGACGTACTTTGACGTCAGCATCTTTCTTGCT GACGGTTGTATCTGTTTCCAATTCACGAGCAGCTTCTTCAGCCATCACATAACTTGCTTTTTGATCATCA AGACGTGGATCGTCATGACGCAAACGCTCTAGTTTGTAATGCGGTGTTTCCAAATGCTTGTTCGGAACCA TCAAGACATTCACTTTGAAGCGAGTCTCAATCTTGATCACTTCAGCACGCTTTTCATTCAAGAGGAACGC AGCCACTTCTACTGGTACTTGAGTATGAATTGCTGCTGTGTTTTCTTTCATTGCCTCTTCTTGAATGATG CGCAAAACTTGCAATGCAGAAGATTCAGTATCGCGAATATGGCCGGTGCCATTACAACGTGGGCAGGTTA CATGGCTACCTTCAGATAGTGCAGGGCGTAAGCGCTGACGGGACATTTCCATCAAGCCAAACTTAGAAAT TTTTCCCATTTGAACCCGGGCGCGGTCATGACGTAGAGCGTCACGTAACCGATTCTCAACATCTTTTTGG GCCTTGCTTGATTCCATGTCGATGAAGTCGATCACGATCAATCCACTCAAGTCACGTAAACGTGCTTGAC GGGCGATTTCATCGGCAGCTTCTAAGTTGGTGCGGGTAGCAGTTTCTTCAATATCAGAGCCGCGTGTTGC GCGTGCTGAGTTCACGTCCACAGAAACCAAAGCTTCCGTGTGGTCGATCACAATTGCGCCGCCAGAAGGC AATGGCACAGTACGTGAGCATGCGGTTTCAATTTGATGCTCAATTTGGAAACGGGAGAACAAAGGCACGT CATCTTGATAGCGCTTCACGCGTGGCAAGTTGTCTGGCATTACAACCGACATAAATGCGGCAGCTTGTTC ATAGATGTCATCGGTATCGATGAGGATCTCGCCGATATCTGGTTGGAAGTAATCGCGAATCGCGCGAATC ACCAAGTTAGATTCGAGGTAGATCAATAGGGGAGCAGAATTGCCTTTGGCTGCTTCATCGATCGCCTTCC ACAATTGCATGAGGTAACTTAAGTCCCACTGCAATTCAGTAGCATCGCGACCGATACCAGCTGTACGAGC AATGATGCTTATGCCGTCTGGCACCTCTAATTGAGACATGGCTTCGCGGAGTTCTTGACGGTCTTCACCC TCAATGCGGCGAGAAACGCCACCTCCACGTGGGTTATTGGGCATTAAGACCAAATAACGGCCTGCCAAAG AGATAAAGGAGGTCAGAGCAGCGCCTTTTTGGCCACGCTCCTCTTTTTCTACTTGAACAATGATTTCTTG ACCTTCGCGCAAAGCGTCCTTAATGGAGGCATTGCGGACGTCGATACCCTCTTTAAAGTAACTGCGGGCA ACTTCCTTAAATGGCAAAAAGCCATGGCGTTCTTCGCCGTAATTGACAAAGCAGGCCTCAAGGGAGGGTT CAATATGGGTAATGACACCTTTGTAGATATTGCCTTTGCGTTGTTCACGGCCGGCAGCTTCGATATCAAT ATCAATGAGTTTTTGACCATCAACGATGGCAACTCGCAACTCTTCTTGTTGAGTTGCATTAAACAACATG CGTTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 350902-353499	Sequence	Pnec_0396 - ribonuclease, Rne/Rng family (Protein is coded in complementary strand; from 353499 to 350902)