Sequence retrieval

Genome: Polynucleobacter necessarius STIR1
Start position: 206141
End position: 206371
Length: 231

DNA sequence
>NC_010531, from 206141 to 206371 (231 bp); Polynucleobacter necessarius STIR1 ATGGCAACTGCCAAGAAAAAACCAGCTGCTAAAAAAGTAGCTGCTAAAAAAGTAGCTAAAAAGCGTCCTG CTGCTAAAAAAGTAGCTGCAAAAAAGGTTGCTAAAAAGCGTCCTGCTGCTAAAAAAGTAGCTGCAAAAAA GGTTGCTAAAAAGCGTCCTGCTGCTAAGAAGCCTGCTGCTAAAAAAGCTGCAAAAAAGGTTGCTAAAAAG CGCGTAGCAAAAAAAAAGTAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 206141-206371	Sequence	Pnec_0224 - hypothetical protein