Sequence retrieval

Genome: Pseudomonas putida GB-1
Start position: 4721745
End position: 4721852
Length: 108

DNA sequence
>NC_010322, from 4721745 to 4721852 (108 bp); Pseudomonas putida GB-1 CTTTTCGACTTTCTCTACCTTTTCTACTTTCTCTACCTTCTCTACCTTCTCTACCTTCTCTACCTTCTCT ACCTTCTCTACCTTCTCTACCTTCTCTACCTTCTCTAC
Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF. 4721590-4723305	Sequence	PputGB1_4221 - hypothetical protein (Protein is coded in complementary strand; from 4723305 to 4721590)