Sequence retrieval

Genome: Ignicoccus hospitalis KIN4/I
Start position: 536474
End position: 537748
Length: 1275

DNA sequence
>NC_009776, from 536474 to 537748 (1275 bp); Ignicoccus hospitalis KIN4/I TTAAGATCGTTCCTCCCTCAACGGGTAACGTCCGGTAAAGCAGCCCATACAAGCCTTGATCGGTATTAGT TTTTCTAACTCCTCCGGCGGGAGCCACTCGATGCTCTTAGCCCCTATATACTCGGCTATCTTTTCGTTGT CCATCTTAGCGGCGATCAGCTGGTCCGCGGGGGGCACCTCCACGCCGTAAGGGCAATGGCTTATTATCTT CGGGCTCCCTATGAGTACGTCGACGCTCACAGCTCCCACCTTGCTCTTTAGGGTTTGTATCACCGTCTTC AAGGTCAGGCCTCTGACCACGCTATCATCAATAATAAGGGCCCTCTTCTTTCTGAAGGTGCTCCTTATCG GGTTCAGCTTCAATTGTACTCCTATCAACCTCTCGCTCAAGTCTGGCTTTATGGCAGTCCTCGCCTTCCT CCCGGTAGCGATAAAGCCCAAGCGGTAGTCTTTCTTGGCCTCCTTAGAGTACCCTATCGCAAATGGTATC GCAGTCTCCGGGACGCCCACGACCACGTCCACGTCCCTCGGGTAGAGCTGGGCGAGCCTCTTGCCTATCC TCTCCCTCACTTCGTAGACCTCTACGCCGTCTATGACCGAGTCCGGCCTCGCGTTGTAGATGTACTCGAA GGCACAGACCACCTTCTCGCTGGGCTTCACCGAAACCTCGTTGAAGTACAGCTGAGAGGCGTAGAAGCCC GTCCCGGGGTCGAGGTCCTTCTTCAAGTCGGCGCCTATGACGTCGAACGCACAGCTCTCGGAGGCGAAGG TGACCATGTCGAAGCCCAAGCCTCCCAGCTGGAGGGGCCTCAGCCCCGGAGGGGAGCGGTAGGCCAAGAG CTCGCCGGAGGCAGTTAAGGCGAGCATGCTAAAGGCTCCCTTTAAGTCCTTCACGAGCTCTTTGAGTAGG TCCTCCCCCTCGTAGCCGCTGGCCTTCAGCTCCGAGAGCTTCTTGGCGACGTCGTAGGGAGTGGCGTTGA ACAGCCTGCCGTCCAAGACTACAGCCGCCTCCGCGGGCTCTTCTGAGTAGGCCCGGTAACCCGGGTGTTC GCTCCACACCCCTCCTATGGCCGCCCAGCCGGCCAAGTTGGTGGCTGGAGGCAAGACCTCGTCGACGAAG CCCTTGCCACAGACCCACTCTATCTTTTCACCGTCGGCGACGTAGAGGCACGCCTCCTCTTGTCCCCTGT GTTGGAGCGAGAGCAAGGAGTAGTACACGAACCTAGCTACGTTCCATACCGGGTCGAAGGCCAGAGCGCC CGCTATTCCGGCCAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 535945-536511	Sequence	Igni_0599 - hypothetical protein
ORF.. 536474-537748	Sequence	Igni_0600 - amidophosphoribosyltransferase (Protein is coded in complementary strand; from 537748 to 536474)