Sequence retrieval

Genome: Mycobacterium bovis BCG str. Pasteur 1173P2
Start position: 2742558
End position: 2747429
Length: 4872

DNA sequence
>NC_008769, from 2742558 to 2747429 (4872 bp); Mycobacterium bovis BCG str. Pasteur 1173P2 TCACCCCGAGATTCCTCGCCCGCTTGTCCGTGTCATGCGGCGGATCTGCCGCGCGGCGACCGACAACGTC GCCAGATCCTTCTGGCCGCTGGCACGGATGTCGTCGAGTGTCCGACGTGCCCGCGCCACCCGGGAGGCGC TGAGGTGTTCCCACTCGGCGATCTTTTGCTCGCTACTCTCGCCGGGTTCCCCCACGGCCAGCACGTCGAA ACACAACGACCGTAGCGCACCGTAAATATCGTCGCGAATCGCCAAGCGCGCCAACGAATGCCAGCGGTCG TGTCGGGGCAGCTGGGATACCGCGGTCAGCAGGCCATCGGTGCCCAGCCGGTCCATCAGGGCGAAATAGG TGTCAGCGACCTCGGCGGCGTCGATGTCGGCGATGTCGGCGATGTCGATGATGTCGAGCAGGCTGTACCG GTACAGGCCGGTCGAGACACGGTAGGCCAAGTCTTCAGGCACACCCTGCGATGCGAATTCCGCAGCTGTC TTTTCGACGATGGCCTTGTCATCACCACGCAACCACTCCGACATGCGCGGTGTCAGTGCCTTGACCATGG CCGCGAATCGGTTGATCTCGGCGCCGACGGCCAAGGGCTGCGGACGGTAGTTGAGCAGCCAGCGTCCGGC ACGGTCGATCAGCCGACGGGTGTCCAGCGTCAACCTGTCTGACAGCGCGATTGGCAGGTTCGCCGCACGG ATCCGGCGCCAAATGTGACCGACACCGAAGATGGCATCGGTGGCGACATAGGTGCGCACGGCATCGATCG GCGTGACACCAACGTCTTCGGCGATCCGGAACGCATAGGTGATGCCGGCGGTATCCACCAGATCGTTGAT CAGCATGGTGGTGACGATCTCGCGGCGCAGCTGGTGGGAACGGATCTCCGGGGTGAACCGTTCGCGCAGC GCCGTCGGGAAATAACGAGGCAACCTGGAAGCGAAGACATCCTGATCCGGTAGTTCGGTGGCTAGCACCT CCTCTTTGAGCCCCAGCTTGACGTGCGCCATCAGCGTGGCGAGTTCGGGCGAGGTGAGCCCGATGCCGGC CTCGGAGCGCCGGGCAATCTCCTTCTCCGACGGCAGCGCTTCCAATTCGCGGTTGACCCCGCGCTCAGCC ACCAAATACTTGATCTGCATTGCGTGCACCGGCAGCAGGCTGGCCGCGTTGGCGCGACTGGTGCCCATCA AGTCGTTCTGATCTTCGTTGTCGGCGAGCACCAGTTGCGCTACCTCGTCGGTCATTGACTCGAGCAGCTG TGTGCGTTCGTCGGCTTTGACCGTGCCGGCGCTCACCAGCGAGTCGATCAGGATCTTGATGTTGACCTCG TGGTCCGAGCAGTCCACGCCGGCGGAGTTGTCCAGCGCGTCGGTGTTGATCCGGCCGCCGGACAGATCGA ATTCGACACGGCCCAACGCCGTCACTCCGAGATTGCCACCTTCGCCAATGACCTTGGCGCGCACTTGATT CGCGTTGACTCGCACCGGATCGTTGGCGCGATCGCCGACATCAGCATCCGACTCTGACTCGGCCTTGATG TAAGTGCCGATGCCGCCGTTGAACAGCAGGTCCACCGGCGCCCGCAGAATCGCCCGAATAAGGTTGGGCG GGGCCATCTCGGCGGCCCCCCCGTCAACTGAGCCGTCGATGCCGAGGACGGCGCGGACCTGCGCGCTGAG CGGGATGGCTTTCTGTTCGCGGCTGTACACCCCGCCGCCCTCGCTGATCAGAGACCTGTCATAGTCGCCC CAGCTGGACCGGGGCAACTCGAACATCCGCCGGCGTTCGGCCCACGACACCGCGGCATCGGGGTTGGGGT CGAGGAAGATGTGGCGGTGGTCGAAGGCGGCGATCAGCCGGATGTGCTTGCTCAGCAACATGCCGTTGCC GAATACGTCGCCGCTCATGTCGCCGATTCCCACGACGGTGAAATCCTGGGTCTGGGTGTCGATCCCGATC TCTCGGAAATGCCGTTTTACGGCCTCCCAGGCCCCCCGGGCGGTGATGCCCATGGCCTTGTGGTCGTAGC CCACCGATCCGCCCGAGGCGAACGCGTCGCCCAGCCAGAACCCATAGGACTTGGCGACATCGTTGGCGAT ATCGGAAAAGGTGGCAGTACCTTTGTCGGCGGCCACTACCAAGTAGGCGTCGTCGCCGTCACGTCGCACC ACCTCGGGCGGGGGGTTGACGCTTGCGGTCGCATGATCGACGTTGTCGGTGACATCGAGCAACCCGGAGA TGAACAGCTGATAGCAGGCGACCCCTTCGGCGCGGGTGGCGTCGCGGTCGGCGGCGGGGTCGCCGGTGGG CAGCGGGGGACGCTTGACCACGAACCCGCCCTTGGCCCCGACCGGCACGATGACGGCGTTCTTCACCGCT TGCGCCTTGACCAATCCGAGAATCTCGGTTCGGAAATCGTCACGGCGGTCCGACCAGCGCAACCCGCCAC GCGCAACTGGGCCGAACCTCAGATGCACGCCTTCGACGCGGGGCGAATACACAAAAATCTCGTACCGGGG ACGCGGCAGCGGAAGTTCGTCGATCAACTGGGCATTGAGTTTCAGCGCCAATACATCACGGCAGCGGGCC GAACCCTGGCGTGTCACAAAGTAATTGGTGCGCAACGTGGCCTGAACCAACGACGCGAAGGCGCGCAGGA TCCGGTCGGTGTCCAGGCTCACCAGCGCGTCGATGTCCGCGGCGACAGCGGCAGCGGCCGCTTGGGCATC GCGATTGCTCGCCGACCCCGACGGCACCGGAACGAAAAGCGCTTCGAACAGATCGACCAAAGACCGAACG GTAGCAGGGTGCTCGTTGAGCACCGATTCAATGTAGGACTGGCTGTACGGGAAGCCCGCCTGGCGCAGGT ACTTCGCGTAGGCACGGAGCAGCACGACCTGCTGCCAAGTCAGCCCGGCACGCATCACCAGCTCGTTGAA TCGGTCGATTTCGACCCGGCCGTGCCAGATCGCGGTCACCGCCTCGGCGAATCGGTGCGCGGTCGCGGCC CGCTCGGCAACCGTCGGGGCCAACGGGATCGTGGGATGCGGCGAGATCTTGAACTGATAGATCCAGACCG GCAGACCGTCCGGCCGGGTGACGGAGAACGGTCGCTCTTCGAGCACCACGACTCCCATGCTTTGCAGCAT CGGCAGCAGCTGGCTCAGCGAAGCGGTGCGCCCACCGAGGAACCAGGTCAACTGGGCGACACCCTGCTCG TCGCGTTCGGAAAACACCAGCTTGACCGAATCGTCGGTCAGCTCCGTGATGACCGCAATGTCGCCAATGG CATCGGCCGGGGTGACGGCCTGTTTGTAGGCCTCGGAGAAGGCGGCAGCGTAATGCATAGCGTCGGCCTG TCCGACGGAGCCAGCCGCCGCCGCGCCGATCAAACGGTCGGCCCAGGTTCGCGCGGCTTCGGTCAGCAGA CCCTGGATCCGGATCCGGTTGGCTTCGGAAACGTCCACCGGCGGGGCGGCCGCCCCTTCTCCTGCCACAC CCACTTCGGGTAGCCGCACCATGAAATGCATGAGTGCCCAAGGTGATTCACTGACCCGAGCGGTGAACTC CAGTCGTGTTCCCCCGAACTCGCGGACAAGGATGTCCTCGAATTGCATGCGCACGGCGGTGGTGTAGCGA TCTCGGGGCATGTAGACCAGGCACGACACGAAGTACTGCAACCGATCCGCGCGCAGGAACAACAACGCCT GCCGTTGCGATCCCAAGTCCACCACGGCCCTGGCCATGGTCAGCAGGCGCTGCGCGCTCAGGGTGAACAG CTCCGGTCGCGGGACGGTCTGGATGACGTCGAGCAGCAATTGGCCTGGGTGGCTGGGATCGCTTTCGGCC ATCGCCAGCGCCTCGCGGACCCGGCGCGAGATCGTCGGGATCTCCAGCACGTCCGCATTCATGGCCGCGA CGCTGAAGAGCCCGACGAAGCGGTGCTCGACCACGCTGCCGTCGACGTATTCGCGGACCGCGATGGCATA GGGATAGGCGCCGTAACGCAGGTAGCTGCCGACCCGCGCTTGGGCCAACACCAGCAGTTTGTCGTCGTCG GTCAGCCGGGGACGCGAACCGGTGCGGCCCCGCAGGACGCCCATACCGCTTGACCCCTCGCCGTAGACCA TCCCGTCAGCCACCCGGCACCGTTGGTAGCCCAGCAGCAGGAAGTTCCCGTCACCCAGCCAACGCAACAG TTCCCCGACGTCTTGTCGGTCGGGCGCGGAAAATCGGCCGCCGGCATTGGATTCGACTTCTCCCGCCAGC TCGCTCAGGGTGGCGATCAGCGCTGTGGCGTCGGTGGCCACCCGCTGGACGTCGGCCAGCACCTTGGGCA GCAACCGCTCCACCTCGGCGAGGCCTTTGTGATCAACGGCGGGCGAGAGCGCTACGTGCATCCAGGCCTC ACCCAGGTGCGGCGACGTGCCCTCGGCCTTCGGTTCGATGCGCAGCAGCTCTCCCGTGGGGCTGCGGTGC ACGTCGAACACCGGGGTCAGAATCGCCGCGTAGGCGATTCCAAGCCGGTGCAGCAGCACCGTAACGGAAT CCATCAGCATGCCGCCGTGCTCGGCGACCACCTGCAGCGCCGGACCGAACCCCGCGGGATCGTCCGCCCG ATAGACGGCGACACAGCTTTCACCGGCCGCGCGGTGCCGGCCAAGCCGATAATGTGCGCCCAGCATGGCG GGCGTCAGCAGGGAGGCTGGAAGCCAACTGGCCTCGGCGGCCTTGGTGGCTTCCGACGAGTCGTCGCGCG GTCCTCGATAGCTGTCGATGTAGGCCTTCGAGATCCAGTCAGGAATGTCCGCACTCGCGGTGAACGTGGT CCACGCCTCAACATCCTGCTTAGCCCCGGGATCGATCGTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 2742145-2742561	Sequence	BCG_2495c - hypothetical protein (Protein is coded in complementary strand; from 2742561 to 2742145)
ORF.. 2742558-2747429	Sequence	BCG_2496c gdh NAD-dependent glutamate dehydrogenase gdh (Protein is coded in complementary strand; from 2747429 to 2742558)