Sequence retrieval

Genome: Mycobacterium sp. KMS
Start position: 512825
End position: 513343
Length: 519

DNA sequence
>NC_008705, from 512825 to 513343 (519 bp); Mycobacterium sp. KMS TCAGCCTCCCGGCGGGACCAGCGTCGGCACCGTGATCGTCACCGTGCTGGTGCTCGTCTCCACCGTCGTG GTGGCCGCAGGCGAGGTGGTGGTCGTGGTCGGCGCCGTCGTCGTGGTGGTCGTCTCGCTGGGTGTGGTGG TCGTGGTCTCGGGCGGCGCCGTCGTCTCCGTCGGCGCCGTCGTCTCGGTCGGCGATTCGGTCACGGTCTG CGGGGGCACCACCGAGGGTGCTTCCGACGTGGTGGTGGTGGTTGTGGTCGTCGTCGTGGATTCGCCCGAG TCGTCGCCCGAGACCAATTGCACGACGAGCCAGACGATCACCGCCACCAGCAGGGCCGCGATCGCACCGA GGGCGATCAGCGCGCCCGGTTTGCGGTACCACGGCTGCGGCTCCCCCGGCGGCGGCTGGTCGTACGCCCC GGTGTAATCGCCGTACTGCGTCGGTTCATCGCTGTAGGCGTTGGAGTACTGGGTCGGCTCGTTGTCCGAA TAATTCGGATCTTCAGGAGGTCGCGCCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 512825-513343	Sequence	Mkms_0460 - hypothetical protein (Protein is coded in complementary strand; from 513343 to 512825)