Sequence retrieval

Genome: Mycobacterium sp. KMS
Start position: 256418
End position: 260572
Length: 4155

DNA sequence
>NC_008705, from 256418 to 260572 (4155 bp); Mycobacterium sp. KMS TCATCGCGGTGCGGAACGGGCGTTGAACGGCACCGCCGAGGCGGCCAGCGCCGCCACCGACACCAGCGCC AGCAACTGCACTCCCGGTTCGAAACCGACGTAGCCGTCCACTGAGCGCCAGGGATGCTGCGAGAGCACGG CGCCGGCGAGGGTCAGCCCGCCCGCCGTGACGCCGAGCGTGAACCGGTCGAGCAGCCGTTCCCGGTTGCG CAGCAGGTAGCGCACCCCGAGGACACCGCCGACGACGACCACCCCGGCCCACCCGGAGATCACTGCCCCG ACCCCCAGGACGCCTACCGCGGCCGCCACCGTGGGCACCCGCCATGGGCGCCCGGGCTCGTCGTCCCGGC GGCGCCGCGCGGGCAGCCAGACCAGCAGCGCGAGCAGCGGGAGCAACGCCAGACCGCCGATCAGCCCGGC GCGGTAGAGCGTGTTCGAGCGGAAGGACAGCGTGACGGCGCCCTCGGTGTCCGCGGGCACCACCCAGCCC TGCTGCCAGCCGTTGACGACGACGGGGGTCAGCAGGTCGCCGCCGGGGCCGCGCGCCTGCCAGCCCGGGT TCACGCTCTCCGGGACCACCAGCACGCGGTCGCGTTGGCCGGGAACGACCCGCACCTCGCGGCGGTCCGG ACCCCACGCGCCGGTGTCGGCGGGCGTCGTGGCCGCGGGTGTGATGTCGCGAGCCGCCGGCGCAGCCAAC TGGACGCCGTCGACCACGAACGTGGCGCCCGGGCTGACGAGGAGTTCCTGCTGTCCGGCGGGTAGCGCGA TCGGCTCGGTGCGGCAGGGTTGCGCGAGCACCGGTTCCCCGTCCACCAGCGCACCGACGGTGGCGGTGAC CCTGGTCGGCACGAATTGTCCGGCGACACCGATGATCGGACCCTCACCGCACGGCACGTCGACGGTCCGG CCGCGGTTCTCGCCGGCGTCGGCGGCGCCGATCGGGTTGCCGCGCCGGTCCAGCGCGGTCACCTCGGCCA GGCCGGGCGGTTTGAGCTGGTCGAACCCGAGCGAGGTGCGGTCGATGATGTCGTTCCAGTCGAGCAGCGA GACCGCGATGGTGTCGGTGCGGTGGGGTGTCAGCCGGATCGTCTCCGTGTCTCCGTCGCCGGAGAGCCGA CGCGCCTGCGGTCCGTTGCCGAGGTCGACGGCCACCATTCGCGGATGGGCGGGCGGCTCGGTCTTGCTGG GCGTGATGCGCAGTGCGGCGACCTCTGTCGGGGCAGGCAGCCTCAGCGTCAGGGTCGGCGGGGTGCGGTG CTGGACGACGCGCTGGGGTGCGGTCCACGCGGTCCGCGGATCGCCGTCGCCCGCGGCGTAGGACGATCCG AGCACGTCGAGGGCGTCGGCGTCCCCGGCGACCACGGTGGTGTCGGGCTGGCGGATCAACTCGGCCAGCC GCGGGCCCTGCCGCGCGCGCACCCACACCGTCGGCTGCACCGACGTCGGTGCGGGTACGGCCAGGGTCCG GCTCAGGTTCACCGGCTCCTCGGGCGACAGGGCCATGGCCGCCGCGCATTTGACGCCGTCCGGACTGTCG ACGCAACCCTGCCGGCCCAGCAGTTCGGAGCCCAGATCCCACAGTCCGACGGCCGAGCCGGCGGGTGGGC CGGGCACCTCCACCGTGTGCCGCAGGTGGATCGGGGTGGCGAATCCGTTGGCGTCGTACTGGGTGACGGA CAGGTCGGTGATGCCGAACTGCACACCGGCCGAACCGTCGTCGGTGCCGGTCGCGGTGATCCGCACCCAC GGGGTCTCGCCGTAGGGCAGCGCGACGGTCAGCGGTTTGCCGGCCTCCTCGAAGCGCAGGGTGCTGGTGC CGTTGACGGTCGCCACCTCCATCCGGCGGACCTGCGCGCCGACGGCGGTGGCGCTGGGGGTGACGGTGAT GGTGGCGTTGGTGACGGGCTTGTCGAAGTCGACCTGCAGCCACTGACCGAGGGCGGCCTGCAGCGCGTTG GACACCCAGCTGGTCGAGGTGTCGGCGTCGATGGCCGCCGTCGGTCCGGTCGCTGGTGCGACGTTGGGCA GCGCGGTCGAATCGGCCGCCGAACTGGACACCGACAACCGTCCGCCGGTCCACCGGCCGTAGACGACGTC TGCGCCCGCCGACGGGTAATCGGGCACCCGGTTGAAGGTGTTGCGCGGGTCGTCGGGGGTGCGGATCGCC GACGAATGGTCGTCGACGCGGCCGTAGTCGGTCTCGCGTGCCGTCGGGGTGTCGGTGACGGTCACCAACG GCGCGGGCAGGCCCGCACGTTCGGCATCCGCGGTGAGCAGCATCGGGCCCAGCGGCGGCCGGTCGAGCAG GCGGCGGCGTTCGTCGAGGCGCAGCAACGCCTCCGGTGCGCCGTCGACCCGTGCCATCGCGTCGGCGTCG ACCAGATACGGAGTCGACGGATTGTGTTGTGCGTCAACGCGATAGATCTCCACCGCCGGGTAGCGGGGCC GCAGTCCGCTGTCGGAGACGAAACCCTCGAGGGTGCCCGGACCGACCGGATCGCCGAACTGCGCGACGCG CGTCAGGCCGGGCGAGCCGTCGATGGCGCGGTGCACCAGCACCGGCCGCGCCGATCGTGACGATTCGGGA TCCAGGTCGTTGCGCACCACGACGTACGAAATTCCCTGGCGGGCAAGAGTGTCCGCGAGCCCGGCGGAGG GCCGGCCCGCGGCGAAGAGGCGCTGCACCGAATCGAGTGCGCGGATCGTCTCCGGCGGGGTCAGCGGAAT CGAATCGCGCACACCCCAGGGGCTCTCACCGAGCACCTGAAGGGGCTCGTCATGGCTGCTGCCCCACACC TGGGTGGCGAACGGTGCGCCGGGCGCCACCAGCACCCGCCCGGCGGTGGGGTCACCGGTGTTGTGGGCGT CCAGCCACGCCGCCGTCTCGTGCCAGTGGCCGGGGATGGCGGTGAACGCGCCCGGCGGTGTGAGCCGGCC CGTCCAGGCCAGTGAGGTGGCCGCGGTGAGCGCGGCGAGGACGACGATGCCGACCGCGACGCGCCGGTCC TTCTCGGGATGGGCGAACGCGTGCAGCCACACCGGTCGCGGCGCGCTGCCCGGCAGGGGGATGCGGGCCA ACAGGTGGATGACGCCCAACGCCAGCGGGAGCCGCAGCACCGGGTCGAGTTTCTGCAGGTTCCGCAGCGG GGTGCCGTCGCCGTCGAGGAAGGCCTGTACCGCGCCCGCGACGGGTGAGCCGAGTCCGCCGGCGTACCCC GCCGCGAGCAACGCGACCCCGACCAGCAGCATCGTGATCAGCCGACCCCGGGCCGGCATCGAACGCAGCG CGAGACCGGCCATCCCCGCCGCGGCCACCAGCGTCGTCGCCAGCACCGCGAGGCTTCCGGTGACCAACGT CGAACCGGCCGTCGCGGTGGGCGCCACGAACGGTGTCCAGCTGCCGGTGCCGCGCAGCATCTCCGTCAGC GACATCCACTGCGTGGTGACGCCGGAGGATTCGATGAAGTCGAGGAACGGTGGGCTGATCCGGCCGAGCA GCAACAGCGGGACCACCCACCACAGCACCGCCAGCGCCGCACAGCCGAACCACCACGCGGTGAACCGCCA CCACAGGCGGTTGGGCCGGTGACACGCCCACCACAGCACCGCGGCCAGGCAGCCGGTGAGCGTGGCCACC GCGTTCACCGCGCCCATCAGCGCGATCGCCCCGGCCGAGCGGGCGGCCAGTCGCCGCGCCGAACCCTCAC CCCGCAGCGCGAGGATGACCGGCAGCAGCACCCACGGCGCCAGCATCATCGGCAGCGTCTCCGACGACAG GGCGCCCAGCGTGGTCAGCACCCGAGGCGACAGCGCATAGGCGACGGCGCCGATCACCCGCGACGTCGGA CTGCCGAGCCCGAGGGCCTCGGCGACCCGCACCACACCCCAGAATCCGACCGTCAGCAGCAACGCCCACC ACAGGCGTTGCGTCACCCACCCGGGCAGCCCCAGGAGGTCACCGGCGAGGAAGAAGGTGCCGTGCGGAAA CAGGTAGCCGTACGCCTGGTTCTGGGCCTGGCCGAACGGCAGCTCGCTGTTCCACAGGTTCGCGGCGCGC GCGAGGAACCGCAGCGGATTCGCGGTGAGGTCGAGTTTGGTGTCGGGGGAGATCTGTCCGGGCGACTGGG CGAAAGTCAGGACGAGCGCCGCCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 254842-256443	Sequence	Mkms_0235 - hypothetical protein
ORF.. 256418-260572	Sequence	Mkms_0236 - hypothetical protein (Protein is coded in complementary strand; from 260572 to 256418)