DNA sequence >NC_007951, from 4012081 to 4012215 (135 bp); Burkholderia xenovorans LB400 chromosome 1 GTGGCGGAAGCAGCGGCGGCGGAAGCAGCGGCGGCGGAAGCAGCGGCGGCGGAAGCAGCGGCGGCGGAAG CAGCGGCGGCGGAAGCAGCGGCGGCGGAAGCAGCGGCGGCGGAAGCAGCGGTGGCGGAAGCAGCG Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI