DNA sequence >NC_007951, from 4012083 to 4012187 (105 bp); Burkholderia xenovorans LB400 chromosome 1 GGCGGAAGCAGCGGCGGCGGAAGCAGCGGCGGCGGAAGCAGCGGCGGCGGAAGCAGCGGCGGCGGAAGCA GCGGCGGCGGAAGCAGCGGCGGCGGAAGCAGCGGC Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI