Sequence retrieval

Genome: Shigella boydii Sb227
Start position: 36831
End position: 38693
Length: 1863

DNA sequence
>NC_007613, from 36830 to 38692 (1863 bp); Shigella boydii Sb227 ATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGG TACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCG ACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATC GGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGA TGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCTGCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATT GATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATG GTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGG CGATGATTCCGCTACTGGCAACGAGCAGTGCCTCGACGACGATGGGCGCATTTGCTCTCTCGGCGTTAAA AGTGGCGGGTGCGCTGGTGCTGGTGGTATTGCTGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTA GCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGC TGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATA CCGTCATGCGCTGGAGAGCGATATCGAACCCTTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTT GGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCC TCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTG GTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAAT GTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCGGCAACGCCGATTC TGCTGGTGATCCTCAATCGCCTTGAGCAATCTTCTACTGAAGAGGCGCGTGAAGCCGATGAGATCGACGA AGAACAGCCGCGGGTAATCATTGCCGGATTCGGTCGTTTTGGGCAGATCACCGGACGTTTACTGCTCTCC AGCGGGGTGAAAATGGTGGTGCTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGA AAGTGTTTTATGGCGATGCCACGCGGATGGATTTACTGGAATCTGCGGGAGCGGCGAAAGCGGAAGTGCT GATTAACGCCATCGACGATCCGCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACACTTCCCGCAT TTGCAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGC CGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGTCTGGGGCC GTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATG GTTGAGAACGACACCAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTA CCGAGGACCGCGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGTAAACATAC CGGCAACATGGCGGATGAACCGGAAACGAAACCCTCATCCTAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 36308-36838	Sequence	SBO_0035 yabF glutathione-regulated potassium-efflux system ancillary protein KefF
ORF.. 36831-38693	Sequence	SBO_0036 kefC glutathione-regulated potassium-efflux system protein KefC