Sequence retrieval

Genome: Pseudomonas syringae pv. syringae B728a
Start position: 574465
End position: 576138
Length: 1674

DNA sequence
>NC_007005, from 574465 to 576138 (1674 bp); Pseudomonas syringae pv. syringae B728a TTACAGCAGGATTGTGCGGATGTCCGCCAGCAATTCGCTCAAGCGCTTGGTGAAGCGGGCAGCAGCTGCG CCGTTGATCACGCGGTGATCATAGGACAGCGACAGCGGCAGCATCAGTTTCGGCTGGAAGGCTTTGCCGT CCCAGACGGGCTGGATGGTTGCCTTGGAAACACCGAGGATCGCCACTTCCGGCGCGTTGACGATCGGCGT GAAGCCGGTGCCGCCAATGTGGCCGAGGCTGGAGATGGTGAAGCACGCGCCCTGCATGTCGTTCGCCGTC AGTTTCTTGTTGCGCGCCTTTTCCGCCAGTGCGGCGGCTTCGGCGGCCAGTTGCAACAGGCTCTTCTGGT CGACGTCGCGGATGACCGGGACCAGCAGGCCGTCCGGGGTGTCCACGGCAAAGCCGATGTGCACGTACTT CTTGCGAATCACGGCCTTGCCGCTAGGCGCCAGCGAAGCGTTGAAGTCCGGCAGCTCCTTGAGCAGGTGG GCGCAGGATTTGAGCAGCAGCGGCAGGACCGTCAGCTTGACGCCAGCCTTCTCGGCAGCGCCTTTCTGCG CCACACGGAAAGCTTCCAGGTCGGTAATGTCGGCCTGGTCGAACTGCGTGACGTGCGGAATGTTCAGCCA GCTGCGGTGCAGGCCGGAAGCGCCGAGTTGCATCAGGCGCGTCATCGGTACTTCTTCGATCTCGCCGAAA CGGCTGAAATCGACTTCCGGAATCGGCGGGATGCCCGAGCCGCCGCTTGCGCCGCCAGCTGGAGCTTCCT TGGCCTTCTGCATCATGGTTTTGACGTAAGCCTGAACGTCTTCTTTCAGCACACGGCCGTGCGGGCCGGT GGCGCTGACAGCGTTCAGCTCGACGCCAAATTCGCGGGCCAGCTGACGAACGGCCGGGCCGGCATGAACC TTGGCACCGTCTTTGGCCGGAGCCGCTGGAGCCGCTGGCGCAGGTGCTGCTTCTGCTTTGGGAGCAGGGG CAGCTGGAGCCGCGGCCTCAGCCTTGGCCGGGGCCGCGGCGGGTGCGCTGGCGGCAGGCGCCGGAGCAGC GGCAGGCGCGGCGCCTTCGACTTTCAGTTTCAGGATCAGGTCGCCGGTACCGACTTCCTGGTCCAGCTTG ACTTCGATGCTCTCGACAACGCCTGCTGCCGGAGAAGGGATCTCCATGCTGGCCTTGTCGGATTCAAGGG TGATCAGCGACTGATCGGCCTGGATGCTGTCACCGACCTTGACCATCAGTTCGATGATCTTGGCCTTGCC CGACGAACCGATGTCCGGTACGTGGATGTCCTGAACGCTGGCGCTGGCTGCCGGAGCAGGCTCGGCGGCG GCCGCAGGTGCAGGTGCCGCTTCAGCCGGCTTTTCGGCAGCAGCAGGGGCAGGGGCAGGGGCAGGGGCAG GGGCAGGTTCGGCAGCAGGGGCCGCAGCCGCAGCCGCTTCGCCTTCCACTTCCAGTTCGAACAGCTCGTC GCCTTCTTTCAGGCGGTCACCCAGCTTGACCTTCATGGTCTTGATGACGCCAGCCTTGGGAGCAGGGATT TCCATGCTCGCCTTGTCCGACTCAAGCGTCAGAACGCTCTGGTCGGCTTCGATGCGATCACCGACCTTGA CCATCAATTCAATGACTTCGCCTTCACCGTTGCCGATGTCGGGTACACGAATTAACTCACTCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 574465-576138	Sequence	Psyr_0517 - dihydrolipoamide acetyltransferase (Protein is coded in complementary strand; from 576138 to 574465)