Sequence retrieval

Genome: Pseudomonas syringae pv. syringae B728a
Start position: 5626435
End position: 5627952
Length: 1518

DNA sequence
>NC_007005, from 5626435 to 5627952 (1518 bp); Pseudomonas syringae pv. syringae B728a TCATGGACGCTCCCGCTCCGCAAACAGTGCCTGAACAAAGGGTTCGGCCTCGAAGGTACGCAGATCATCG ATACCCTCACCGACGCCGATATAGCGAATTGGCAAGCCGAACTGCTTGGCCAGGGCGAAAATCACGCCGC CCTTGGCGGTGCCATCCAGCTTGGTCAGCGCCAGCCCGGTCAGCGTGACGGTCTGGTTGAACTGCTTGGC CTGATTGATAGCGTTCTGCCCGGTGCCCGCATCGAGGACCAGCAGGACTTCGTGAGGAGCGTCTGCGTCC AGCTTGCCGATCACGCGGCGAACCTTCTTCAACTCCTCCATCAGGTTGTCCTTGGTGTGCAGACGGCCAG CGGTGTCGGCGATCAGGACGTCGATGCCACGAGCCTTGGCGGCCTGCACGGCGTCGAAGATGACAGAAGC CGAGTCGGCACCGGTGTGCTGGGCGATGACCGGTATGTGATTACGCTCACCCCAGACCTGCAATTGCTCG ACGGCTGCAGCACGGAATGTGTCGCCCGCCGCCAGCATGACCTTCTTGCCTTCCAGTTGCAGCTTCTTGG CCAGCTTGCCAATGGTCGTGGTCTTGCCCGCGCCATTGACGCCGACCACCAGAATCACGAACGGCTTGTG TTCGGCCTTGATCACCAGCGGTTGCTCGACAGGCTTGAGCATCGCGGCCAACTCACCCTGCAACGAGGTG TACAGTGCCTGGGCGTCGGTCAACTGCTTGCGCGCGACCTTCTGGGTCAGGCTCTTGATGATGACCGAGG TGGCTTCAACGCCGACATCGGCGGTCAGCAGACGGGTTTCGATTTCTTCGAGCAGGTCGTCATCGATGGC CTTCTTGCCCAGAAACAGGCTGGCCATGCCTTCGCCGATGCTGGCACTGGTCTTCGACAAGCCCTGCTTG AGGCGTGCAAAGAAGCCGACCTTGCCGGTTTCTGACTCGGGTGCCGGAGCAGGCACTGCAAGCGCCTGCG CAGCAGGCTGAACCGGTTGCGGCACTGCCTGCGCGACGACTGGCTCTGGCTCTGGCTCTGGCTCTGGCTC TGCAACAGGCTGAACCAGCACCGGCTCAGGCATCCCGAGCGGCACGATATCCGCCACAGGCTCCGCTACG ACGGGTTCAGGCTCCAGCGGCTCGGGCGCAACAGGGGCATGGCTTTGTTCAGGGATGGCCGGCGTGACAT GCGGCTCGCGCTCGTCGCTCAAGGCCACCGGCTCTTCGGTCACCGGCAAGGTCAGCCAGGGCTGAGGCGA AGGCGCACCGGGCTCCACATGCAGTTGCGATGCAGGCGCAGCTGCTTCGACAGTGACTTCAGGCACCGAT TGCGAGGCCACATGAGGCTCAACCTGCGGCGTCGCGGCAACCTGATCGGCGATCGGGTCGGGTTGCGTCG GGGATGATTCTGCTGGCTTCGGCTCGGTTTGCTGCGGCTTCTTGCGCAGCCATCCGAACAGGCCTTTCTT CTCTCCAGCCTCGGCTGGGGTCTTCTTGTCGTCGTTGGAACCAAACAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 5625767-5626438	Sequence	Psyr_4750 - ABC transporter (Protein is coded in complementary strand; from 5626438 to 5625767)
ORF.. 5626435-5627952	Sequence	Psyr_4751 - cell division transporter substrate-binding protein FtsY (Protein is coded in complementary strand; from 5627952 to 5626435)