Sequence retrieval

Genome: Pseudomonas syringae pv. syringae B728a
Start position: 1199033
End position: 1200517
Length: 1485

DNA sequence
>NC_007005, from 1199033 to 1200517 (1485 bp); Pseudomonas syringae pv. syringae B728a TCAGAAGCGCCAGATGACGTCGACAACAGCGCGGTGCATGTAGCTGTCCACGTTGTTCTGATACGCGTCG CCTGGCTTGAAGACACCTGCGCGCAGGCGCACCAGTGCCGAAGGTTCATCGAACGACTGGCTCAGCGAAG CCGGCAGCAGGCCTTGCTTGAAGTACTTGGTCACGACCAGGTCCATTTCCTGACCCAGGTCCTTGCTGCC GTCTTCCAGTGGCAGCGAGCTGAACGTACCGTCCTGGTTGTCGTTCACCGCGTTGATGCCCGAGCCGCCG ATACCGGTGTTGCCGTCCACGCGACGGAACTTGTGGTAGATCAGCGATGCGTCGTATTCCTCGTTCATCT GCCACGAAGCGAACAGCGAGCCGGTTTCCACGTTGGCCATTTCGCCCTGGAAGGCCTCGCCGAAGCGATG GATGCGCGAACGCGTGCCGGTCCAGTTGGAGCGGTTACTTTCCAGACCGTTCTGGACGTAGTCCTTGCTG GCGCGCGAGTAGGCGGCGCCGACTTGCCATTGCGGGTCCAGGCGCAGGCGCAGGCCCAGGTCGGTGGCCC AGCCATTCATGTCACGGCTGTTCTTTTCGCCGGCGATGAACTGGTCGTTGGGAGCAGAGGTCACGCCGAT TTCGTCGCGCGTTCCGTTCAGCCAGGTCAGGCTGCCCCAGTAGTTGAGCTGATTGGTGTTGCTGCGATAG TTGTAGGCATCGCTGTCAGCCTGCAGGCCGACCCAGGTCAGATCGCCATTGGACGTCTTGTCCAGATCAT CCAGCACCTGGCCCTGCGACTTGAGCTTGCCGTCGTCAGCACTGTGGTGAGCGCGGATACCGGCCCAGTT GCCTGGTGTCCACTGATAGCTGACCGAGCCGAACAGGTGTTTGCGATCTTCATCGTCAGGTGCCAGTTCG GTCAGGTCGGTGCGGTATTCGCTGAAACGCTGGGCAGCACCCAGTTCAGCGCGCAACAGTGTGGTATCGA ACGACCAGTTGATGGCCTCGATGTTGGTGTCGTGCCATTGACCGTCGTCGTTGCGCAGACGCTGACGACC GATTTTCAGGATCTCGCCAGGGTAGGGCGTGAAGCCGCTGTAGCCGATCCAGAACTCACGCAGTGCCGCG TAGGTTTTGTCGGCTTCGCGCTCGCTGGCGGTGCCACGCGAAGTCTGGGTGCCGCTGCCGTCGCTGTTGC TCTGCTCCAGCGGGTCGGTCTGAATGATGTCGGTGGCGGTGACCACCTGGCCCATCGCGTAACCGCTCCA GTCGCCGCGCTCGCCATAGACCCAGGGACGCAGGTCCAGGCCCACACCGTTGACGTCGCCGCCCGAACGG GTGCCCAGATCGCGATCATCCTCGGCCTGCGCCGTGGCTTTGATTTCGATACCGAAATTCTGCGGTTGTT CGGTCAGCGCTGCCAGCGTCGGGCAGGCCCACAACAGGGTGAAGCCAAGGCCCATGCCGGCGGCCATCAG GGGATTCAACTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 1199033-1200517	Sequence	Psyr_1059 - alginate biosynthesis protein AlgE (Protein is coded in complementary strand; from 1200517 to 1199033)
ORF... 1200514-1201926	Sequence	Psyr_1060 - Sel1 repeat-containing protein (Protein is coded in complementary strand; from 1201926 to 1200514)