Sequence retrieval

Genome: Vibrio vulnificus YJ016
Start position: 459740
End position: 460228
Length: 489

DNA sequence
>NC_005139, from 459740 to 460228 (489 bp); Vibrio vulnificus YJ016 ATGATCATCAGTATGATTGCCGCGATGGCAAAGCAAAGAATCATTGGTAAAGATAACCAGATGCCTTGGC ATCTTCCCGCAGATTTTGCTTGGTTCAAACGCTGCACGATGGGGAAACCGGTCGTGATGGGGCGTAAAAC GTATGATTCGATCGGACGTCCGTTGCCAGGGCGATTGAATATAGTGATTAGTCGTGACGAAAGCTTGGTT ATTGAGGGGGTCACTATGGCCAACTCGATTGCGCATGCCTTAGCGTTAGCGGGGGATGCAGATGAAGTGA TGATCATCGGTGGCGGTTCAATTTACGCTGAGTGTTTACCAAAAGCAGACAAACTCTATCTGACGTTTAT CGATGCCACTATTGATGGCGATACTCAGTTTCCTGATTGGGGGGAGCAATGGTGCGAGAGCCATCGCGAA CACTATTCTGCTGATGAGAAAAATCGCTACGCGATGGATTTTGTTATTTTAGAAAGAGATAATTGCTAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 459740-460228	Sequence	VV0475 - dihydrofolate reductase